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Journal of Biomedical Optics

Use of picosecond Kerr-gated Raman spectroscopy to suppress signals from both surface and deep layers in bladder and prostate tissue
Author(s): Maria Consuelo Hart Prieto; Pavel Matousek; Michael Towrie; Anthony William Parker; Mark P.J. Wright; Alastair Ritchie; Nicholas Stone
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Paper Abstract

Raman spectroscopy is an optical technique able to interrogate biological tissues, giving us an understanding of the changes in molecular structure that are associated with disease development. The Kerr-gated Raman spectroscopy technique uses a picosecond pulsed laser as well as fast temporal gating of collected Raman scattered light. Prostate samples for this study were obtained by taking a chip at the transurethral resection of the prostate (TURP), and bladder samples from a biopsy taken at transurethral resection of bladder tumor (TURBT) and TURP. Spectra obtained through the bladder and prostate gland tissue, at different time delays after the laser pulse, clearly show change in the spectra as depth profiling occurs, eventually showing signals from the uric acid cell and urea cell, respectively. We show for the first time, using this novel technique, that we are able to obtain spectra from different depths through both the prostate gland and the bladder. This has major implications in the future of Raman spectroscopy as a tool for diagnosis. With the help of Raman spectroscopy and Kerr gating, it may be possible to pick up the spectral differences from a small focus of adenocarcinoma of the prostate gland in an otherwise benign gland, and also stage the bladder cancers by assessing the base of the tumor post resection.

Paper Details

Date Published: 1 July 2005
PDF: 6 pages
J. Biomed. Opt. 10(4) 044006 doi: 10.1117/1.1991848
Published in: Journal of Biomedical Optics Volume 10, Issue 4
Show Author Affiliations
Maria Consuelo Hart Prieto, Gloucestershire Hospitals NHS Foundation Trust (United Kingdom)
Pavel Matousek, Rutherford Appleton Lab. (United Kingdom)
Michael Towrie, Rutherford Appleton Lab. (United Kingdom)
Anthony William Parker, Rutherford Appleton Lab. (United Kingdom)
Mark P.J. Wright, United Bristol Healthcare NHS Trust (United Kingdom)
Alastair Ritchie, Gloucestershire Hospitals NHS Trust (United Kingdom)
Nicholas Stone, Gloucestershire Royal Hospital (United Kingdom)

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