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Journal of Biomedical Optics

Biochemical differences in tumorigenic and nontumorigenic cells measured by Raman and infrared spectroscopy
Author(s): Judith R. Mourant; Kurt W. Short; Susan Carpenter; Nagapratima Kunapareddy; Leslie Coburn; Tamara Powers; James P. Freyer
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Paper Abstract

Both infrared and Raman spectroscopies have the potential to noninvasively estimate the biochemical composition of mammalian cells, although this cannot be unambiguously determined from analysis approaches such as peak assignment or multivariate classification methods. We have developed a fitting routine that determines biochemical composition using basis spectra for the major types of biochemicals found in mammalian cells (protein, DNA, RNA, lipid and glycogen), which is shown to be robust and reproducible. We measured both infrared and Raman spectra of viable suspensions of pairs of nontumorigenic and tumorigenic rat fibroblast cell lines. To model in vivo conditions, we compared nonproliferating, nontumorigenic cells to proliferating, tumorigenic cells. Reproducible differences in biochemical composition were found for both nontumorigenic/tumorigenic cell models, using both spectroscopic techniques. These included an increased fraction of protein and nucleic acids in the tumorigenic cells, with a corresponding decrease in lipid and glycogen fractions. Measurements of each cell type in both the proliferating and nonproliferating states showed that proliferative status was the major determinant of differences in vibrational spectra, rather than tumorigenicity per se. The smallness of the spectral changes associated with tumorgenicity may be due to the subtle nature of the oncogenic change in this system (a single mutant oncogene).

Paper Details

Date Published: 1 May 2005
PDF: 15 pages
J. Biomed. Opt. 10(3) 031106 doi: 10.1117/1.1928050
Published in: Journal of Biomedical Optics Volume 10, Issue 3
Show Author Affiliations
Judith R. Mourant, Los Alamos National Lab. (United States)
Kurt W. Short, Los Alamos National Lab. (United States)
Susan Carpenter, Los Alamos National Lab. (United States)
Nagapratima Kunapareddy, Boston Univ. (United States)
Leslie Coburn, Los Alamos National Lab. (United States)
Tamara Powers, Los Alamos National Lab. (United States)
James P. Freyer, Los Alamos National Lab. (United States)

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