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Journal of Biomedical Optics

Bispectral fluorescence imaging combined with texture analysis and linear discrimination for correlation with histopathologic extent of basal cell carcinoma
Author(s): Marica B. Ericson; Jesper Uhre; Charlotta Berndtsson; Bo Stenquist; Olle Larkö; Ann-Marie K. Wennberg; Arne Rosén
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Paper Abstract

Fluorescence imaging has been shown to be a potential complement to visual inspection for demarcation of basal cell carcinoma (BCC), which is the most common type of skin cancer. Earlier studies have shown promising results when combining autofluorescence with protoporphyrin IX (Pp IX) fluorescence, induced by application of δ-5-aminolaevulinic acid (ALA). In this work, we have tried to further improve the ability of this technique to discriminate between areas of tumor and normal skin by implementing texture analysis and Fisher linear discrimination (FLD) on bispectral fluorescence data of BCCs located on the face. Classification maps of the lesions have been obtained from histopathologic mapping of the excised tumors. The contrast feature obtained from co-occurrence matrices was found to provide useful information, particularly for the ALA-induced Pp IX fluorescence data. Moreover, the neighborhood average features of both autofluorescence and Pp IX fluorescence were preferentially included in the analysis. The algorithm was trained by using a training set of images with good agreement with histopathology, which improved the discriminability of the validation set. In addition, cross validation of the training set showed good discriminability. Our results imply that FLD and texture analysis are preferential for correlation between bispectral fluorescence images and the histopathologic extension of the tumors.

Paper Details

Date Published: 1 May 2005
PDF: 8 pages
J. Biomed. Opt. 10(3) 034009 doi: 10.1117/1.1925650
Published in: Journal of Biomedical Optics Volume 10, Issue 3
Show Author Affiliations
Marica B. Ericson, Sahlgrenska Univ. Hospital (Sweden)
Jesper Uhre, Chalmers Tekniska Högskola (Sweden)
Charlotta Berndtsson, Chalmers Tekniska Högskola (Sweden)
Bo Stenquist, Sahlgrenska Univ. Hospital (Sweden)
Olle Larkö, Sahlgrenska Univ. Hospital (Sweden)
Ann-Marie K. Wennberg, Sahlgrenska Univ. Hospital (Sweden)
Arne Rosén, Chalmers Tekniska Högskola (Sweden)

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