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Journal of Biomedical Optics

Surface plasmon resonance phase-shift interferometry: Real-time DNA microarray hybridization analysis
Author(s): Shean-Jen Chen; Yuan-Deng Su; Feng-Ming Hsiu; Chia-Yuan Tsou; Yi-Kuang Chen
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Paper Abstract

Surface plasmon resonance (SPR) phase-shift interferometry (PSI) is a novel technique which combines SPR and modified Mach–Zehnder PSI to measure the spatial phase variation caused by biomolecular interactions upon a sensing chip. The SPR-PSI imaging system offers high resolution and high-throughout screening capabilities for microarray DNA hybridization without the need for additional labeling, and provides valuable quantitative information. The SPR-PSI imaging system has an enhanced detection limit of 2.5×10–7 refraction index change, a long-term phase stability of π/100 in 30 min, and a spatial phase resolution of π/300 with 100×100 µm2 detection area. This study successfully demonstrates the label-free observation of 15-mer DNA microarray.

Paper Details

Date Published: 1 May 2005
PDF: 6 pages
J. Biomed. Opt. 10(3) 034005 doi: 10.1117/1.1924713
Published in: Journal of Biomedical Optics Volume 10, Issue 3
Show Author Affiliations
Shean-Jen Chen, National Cheng Kung Univ. (Taiwan)
Yuan-Deng Su, National Cheng Kung Univ. (Taiwan)
Feng-Ming Hsiu, National Central Univ. (Taiwan)
Chia-Yuan Tsou, National Central Univ. (Taiwan)
Yi-Kuang Chen, National Central Univ. (Taiwan)

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