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Journal of Biomedical Optics

Tissue characterization using high wave number Raman spectroscopy
Author(s): Senada Koljenovic; Tom C. Bakker Schut; Rolf Wolthuis; B. de Jong; L. Santos; Peter J. Caspers; Johan M. Kros; Gerwin J. Puppels
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Paper Abstract

Raman spectroscopy is a powerful diagnostic tool, enabling tissue identification and classification. Mostly, the so-called fingerprint (~400–1800 cm–1) spectral region is used. In vivo application often requires small flexible fiber-optic probes, and is hindered by the intense Raman signal that is generated in the fused silica core of the fiber. This necessitates filtering of laser light, which is guided to the tissue, and of the scattered light collected from the tissue, leading to complex and expensive designs. Fused silica has no Raman signal in the high wave number region (2400–3800 cm–1). This enables the use of a single unfiltered fiber to guide laser light to the tissue and to collect scattered light in this spectral region. We show, by means of a comparison of in vitro Raman microspectroscopic maps of thin tissue sections (brain tumors, bladder), measured both in the high wave number region and in the fingerprint region, that essentially the same diagnostic information is obtained in the two wave number regions. This suggests that for many clinical applications the technological hurdle of designing and constructing suitable fiber-optic probes may be eliminated by using the high wave number region and a simple piece of standard optical fiber.

Paper Details

Date Published: 1 May 2005
PDF: 11 pages
J. Biomed. Opt. 10(3) 031116 doi: 10.1117/1.1922307
Published in: Journal of Biomedical Optics Volume 10, Issue 3
Show Author Affiliations
Senada Koljenovic, Erasmus Medical Ctr. (Netherlands)
Tom C. Bakker Schut, Erasmus Medical Ctr. (Netherlands)
Rolf Wolthuis, Erasmus Medical Ctr. (Netherlands)
B. de Jong, Erasmus Medical Ctr. (Netherlands)
L. Santos, Erasmus Medical Ctr. (Netherlands)
Peter J. Caspers, Erasmus Medical Ctr. (Netherlands)
Johan M. Kros, Erasmus Medical Ctr. (Netherlands)
Gerwin J. Puppels, Erasmus Medical Ctr. (Netherlands)

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