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Journal of Biomedical Optics

Photodynamic therapy for the treatment of metastatic lesions in bone: Studies in rat and porcine models
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Paper Abstract

This study represents the first reported use of photodynamic therapy (PDT) for metastatic bone lesions and specifically, as a treatment for spinal metastases. A model of bone metastasis in rat confirmed the efficacy of benzoporphyrin derivative-monoacid-mediated PDT for treating lesions within the spine and appendicular bone. Fluorimetry confirmed the selective accumulation of drug into the tumor(s) at 3 h post-injection. 48 h post-light delivery into the vertebral body of the rat spine loss of bioluminescent signal and histological analyses of sectioned spine confirmed MT-1 tumor cell kill in vivo as previously confirmed in vitro using an established cell viability assay. Porcine vertebrae provided a model comparable to that of human for light propagation and PDT response. Histological examination of vertebrae 48 h post-PDT revealed a necrotic radius of 0.6 cm with an average fluence rate of 4.3 mW/cm2. Non-necrotic tissue damage was evident up to 2 cm out from the treatment fiber. Results support the application of PDT to the treatment of primary or metastatic lesions within bone.

Paper Details

Date Published: 1 May 2005
PDF: 13 pages
J. Biomed. Opt. 10(3) 034011 doi: 10.1117/1.1921887
Published in: Journal of Biomedical Optics Volume 10, Issue 3
Show Author Affiliations
Shane Burch, Univ. of California/San Francisco (United States)
Arjun Bogaards, Univ. of Toronto (Canada)
Jeffrey H. Siewerdsen, Univ. of Toronto (Canada)
Douglas J. Moseley, Princess Margaret Hospital (Canada)
Albert Yee, Sunnybrook and Women's Health Sciences Ctr. (Canada)
J. Finkelstein, Sunnybrook and Women's College Health Sciences Ctr (Canada)
Robert A. Weersink, Univ. of Toronto (Canada)
Brian C. Wilson, Univ. of Toronto (Canada)
Stuart K. Bisland, Princess Margaret Hospital (Canada)

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