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Journal of Biomedical Optics

Analysis of biofluids in aqueous environment based on mid-infrared spectroscopy
Author(s): Heinz Fabian; Peter Lasch; Dieter Naumann
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Paper Abstract

In this study we describe a semiautomatic Fourier transform infrared spectroscopic methodology for the analysis of liquid serum samples, which combines simple sample introduction with high sample throughput. The applicability of this new infrared technology to the analysis of liquid serum samples from a cohort of cattle naturally infected with bovine spongiform encephalopathy and from controls was explored in comparison to the conventional approach based on transmission infrared spectroscopy of dried serum films. Artifical neural network analysis of the infrared data was performed to differentiate between bovine spongiform encephalopathy-negative controls and animals in the late stage of the disease. After training of artifical neural network classifiers, infrared spectra of sera from an independent external validation data set were analyzed. In this way, sensitivities between 90 and 96% and specificities between 84 and 92% were achieved, respectively, depending upon the strategy of data collection and data analysis. Based on these results, the advantages and limitations of the liquid sample technique and the dried film approach for routine analysis of biofluids are discussed.

Paper Details

Date Published: 1 May 2005
PDF: 10 pages
J. Biomed. Opt. 10(3) 031103 doi: 10.1117/1.1917844
Published in: Journal of Biomedical Optics Volume 10, Issue 3
Show Author Affiliations
Heinz Fabian, Robert Koch-Institut (Germany)
Peter Lasch, Robert Koch-Institut (Germany)
Dieter Naumann, Robert Koch-Institut (Germany)


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