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Journal of Biomedical Optics

Remote temperature monitoring in ocular tissue using confocal Raman spectroscopy
Author(s): Noel J.C. Bauer; Massoud Motamedi; Fred Hendrikse; James P. Wicksted
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Paper Abstract

We demonstrated the feasibility of Raman spectroscopy for remote temperature monitoring within the aqueous humor of the rabbit eye in vivo. Using a confocal Raman spectroscopy system, Raman spectra from 2580 to 3800 cm–1 were recorded in HPLC-grade water and in the aqueous humor of the rabbit eye under in vivo and ex vivo conditions within a temperature range of 14–34 °C. The ratio between the integrated Raman intensities of two temperature dependent OH-vibrational regions (OH2/OH1) in the spectra of water showed high linear dependence on temperature both in pure water [0.0049(±1.2%)T+0.4522(±0.5%), R2=0.99, n=50, p<0.05], as well as in the rabbit aqueous humor [0.0036(±2.8%)T+0.4966(±0.6%), R2=0.98, n=162, p<0.05] with a high degree of reproducibility and sensitivity (~0.2–0.7 °C). Raman spectroscopy can be used for high resolution and remote monitoring of temperature in the aqueous humor under in vivo conditions.

Paper Details

Date Published: 1 May 2005
PDF: 5 pages
J. Biomed. Opt. 10(3) 031109 doi: 10.1117/1.1911901
Published in: Journal of Biomedical Optics Volume 10, Issue 3
Show Author Affiliations
Noel J.C. Bauer, The Univ. of Texas Medical Branch at Galveston (United States)
Massoud Motamedi, The Univ. of Texas Medical Branch at Galveston (United States)
Fred Hendrikse, Academic Hospital Maastricht (Netherlands)
James P. Wicksted, Oklahoma State Univ. (United States)

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