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Journal of Biomedical Optics

Raman spectroscopy study of atherosclerosis in human carotid artery
Author(s): Grazielle Vilela Nogueira; Landulfo Silveira Silveira; Airton Abrahao Martin; Renato Amaro Zângaro; Marcos Tadeu Tavares Pacheco; Maria Cristina Chavantes; Carlos Augusto Pasqualucci
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Paper Abstract

Fourier-transform (FT)-Raman spectroscopy has been used for identification and evaluation of human artherosclerotic lesions, providing biochemical information on arteries. In this work, fragments of human carotid arteries postmortem were analyzed using a FT-Raman spectrometer operating at an excitation wavelength of 1064 nm, power of 200 mW, and spectral resolution of 4 cm–1. A total of 75 carotid fragments were spectroscopically scanned and FT-Raman results were compared with histopathology. Discriminant analysis using Mahalanobis distance was applied over principal components scores for tissue classification into three categories: nonatherosclerotic, atherosclerotic plaque without calcification and with calcification. Nonatherosclerotic artery, atherosclerotic plaque, and calcified plaque exhibit spectral signatures related to biochemicals presented in each tissue type, such as bands of collagen and elastin (proteins), cholesterol and its esters, and calcium hydroxyapatite and carbonate apatite, respectively. Spectra of nonatherosclerotic artery were then classified into two groups: normal and discrete diffuse thickening of the intima layer (first group) and moderate and intense diffuse thickening of the intima layer (second group). FT-Raman could identify and classify the tissues found in the atherosclerotic process in human carotid in vitro and had the ability to identify alterations to the diffuse thickening of the intima layer and classify it depending on the intensity of the thickening.

Paper Details

Date Published: 1 May 2005
PDF: 7 pages
J. Biomed. Opt. 10(3) 031117 doi: 10.1117/1.1908129
Published in: Journal of Biomedical Optics Volume 10, Issue 3
Show Author Affiliations
Grazielle Vilela Nogueira, Univ. do Vale do Paráiba (Brazil)
Landulfo Silveira Silveira, Univ. do Vale do Paráiba (Brazil)
Airton Abrahao Martin, Univ. do Vale do Paráiba (Brazil)
Renato Amaro Zângaro, Univ. do Vale do Paráiba (Brazil)
Marcos Tadeu Tavares Pacheco, Univ. do Vale do Paráiba (Brazil)
Maria Cristina Chavantes, Univ. do Vale de Sao Paulo (Brazil)
Carlos Augusto Pasqualucci, Univ. de Sao Paulo (Brazil)

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