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Journal of Biomedical Optics • Open Access

Imaging of cardiovascular structures using near-infrared femtosecond multiphoton laser scanning microscopy
Author(s): Katja Schenke-Layland; Iris Riemann; Ulrich A. Stock; Karsten König

Paper Abstract

Multiphoton imaging represents a novel and very promising medical diagnostic technology for the high-resolution analysis of living biological tissues. We performed multiphoton imaging to analyzed structural features of extracellular matrix (ECM) components, e.g., collagen and elastin, of vital pulmonary and aortic heart valves. High-resolution autofluorescence images of collagenous and elastic fibers were demonstrated using multifluorophore, multiphoton excitation at two different wavelengths and optical sectioning, without the requirement of embedding, fixation, or staining. Collagenous structures were selectively imaged by detection of second harmonic generation (SHG). Additionally, routine histology and electron microscopy were integrated to verify the observed results. In comparison with pulmonary tissues, aortic heart valve specimens show very similar matrix formations. The quality of the resulting three-dimensional (3-D) images enabled the differentiation between collagenous and elastic fibers. These experimental results indicate that multiphoton imaging with near-infrared (NIR) femtosecond laser pulses may prove to be a useful tool for the nondestructive monitoring and characterization of cardiovascular structures.

Paper Details

Date Published: 1 March 2005
PDF: 5 pages
J. Biomed. Opt. 10(2) 024017 doi: 10.1117/1.1896966
Published in: Journal of Biomedical Optics Volume 10, Issue 2
Show Author Affiliations
Katja Schenke-Layland, Friedrich-Schiller-Univ. Jena (Germany)
Iris Riemann, Fraunhofer-Institut für Biomedizinische Technik (Germany)
Ulrich A. Stock, Friedrich-Schiller-Univ. Jena (Germany)
Karsten König, Fraunhofer-Institut für Biomedizinische Technik (Germany)

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