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Journal of Biomedical Optics

Two-photon 3-D mapping of ex vivo human skin endogenous fluorescence species based on fluorescence emission spectra
Author(s): Lily Laiho Hsu; Serge Blaise Pelet; Thomas M. Hancewicz; Peter D. Kaplan; Peter T. C. So
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Paper Abstract

Spectral resolved tissue imaging has a broad range of biomedical applications such as the minimally invasive diagnosis of diseases and the study of wound healing and tissue engineering processes. Two-photon microscopy imaging of endogenous fluorescence has been shown to be a powerful method for the quantification of tissue structure and biochemistry. While two-photon excited autofluorescence is observed ubiquitously, the identities and distributions of endogenous fluorophores have not been completely characterized in most tissues. We develop an image-guided spectral analysis method to analyze the distribution of fluorophores in human skin from 3-D resolved two-photon images. We identify five factors that contribute to most of the luminescence signals from human skin. Luminescence species identified include tryptophan, NAD(P)H, melanin, and elastin, which are autofluorescent, and collagen that contributes to a second harmonic signal.

Paper Details

Date Published: 1 March 2005
PDF: 10 pages
J. Biomed. Opt. 10(2) 024016 doi: 10.1117/1.1891370
Published in: Journal of Biomedical Optics Volume 10, Issue 2
Show Author Affiliations
Lily Laiho Hsu, Massachusetts Institute of Technology (United States)
Serge Blaise Pelet, Massachusetts Institute of Technology (United States)
Thomas M. Hancewicz, Unilever Research & Development (United States)
Peter D. Kaplan, Unilever Research US, Inc. (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)

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