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Journal of Biomedical Optics

Hemoglobin adsorption isotherm at the silica-water interface with evanescent wave cavity ring-down spectroscopy
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Paper Abstract

Evanescent wave cavity ring-down spectroscopy (EW-CRDS) is used to observe the adsorption isotherm for hemoglobin (Hb) from controlled urine samples to assess the potential for rapid diagnosis in hemoglobinuria. The absorbance of Hb at 425 nm is monitored using an alexandrite laser-pumped, room temperature, LiF:F2+** color-center pulsed laser. A minimum absorbance detection level of 2.57×10–4 is achieved, corresponding to a minimum detectable concentration of Hb in urea of 5.8 nM. A multilayered Hb biofilm is formed, and a minimum of eight layers are required to model the adsorption isotherm, allowing for cooperative binding within the layers and extending 56 nm into the interface. A binding constant for Hb to silica 18.23±7.58×106 M is derived, and a binding constant for Hb to Hb in subsequent layers is determined to be 5.631±0.432×105 M. Stoichiometric binding coefficients of 1.530±0.981 for layer one and 1.792±0.162 for subsequent layers suggest that cooperative binding both to the silica surface and between the layers of the biofilm is important.

Paper Details

Date Published: 1 March 2005
PDF: 7 pages
J. Biomed. Opt. 10(2) 024025 doi: 10.1117/1.1891368
Published in: Journal of Biomedical Optics Volume 10, Issue 2
Show Author Affiliations
William Blake Martin, Medicraft, Inc. (United States)
Sergey B. Mirov, The Univ. of Alabama at Birmingham (United States)
Dmitri V. Martyshkin, The Univ. of Alabama at Birmingham (United States)
Ramakrishna Venugopalan, The Univ. of Alabama at Birmingham (United States)
Andrew M. Shaw, Univ. of Exeter (United Kingdom)

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