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Journal of Biomedical Optics

In situ fluorescence imaging of organs through compact scanning head for confocal laser microscopy
Author(s): Taisuke Ota; Hiroya Fukuyama; Yasuhige Ishihara; Hideo Tanaka; Tetsuro Takamatsu
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Paper Abstract

We develop a compact scanning head for use in laser confocal fluorescence microscopy for in situ fluorescence imaging of organs. The head, cylindrical in shape, has 3.5 mm diameter and 30 mm length, and is thus small enough to operate in a living rat heart. The lateral and axial resolutions, defined as full widths at half maximum (FWHM) of a point spread function (PSF), measures 1.0 and 5.0 µm, respectively, for 488-nm excitation and 1.0 and 5.4 µm, respectively, for 543-nm excitation. The chromatic aberration between 488- and 543-nm laser beams is well suppressed. We perform Ca2+ imaging in cardiomyocytes through the right ventricular chamber of a perfused rat heart in line-scan mode with 2.9-ms time resolution. We also carried out two-color imaging of a fixed mouse heart and liver with subcellular resolution. The compact head of the microscope equipped with a line-scan imaging mode and two-color imaging mode is useful for in situ imaging in living organs with subcellular resolution and can advantageously be applied to in vivo research.

Paper Details

Date Published: 1 March 2005
PDF: 4 pages
J. Biomed. Opt. 10(2) 024010 doi: 10.1117/1.1890411
Published in: Journal of Biomedical Optics Volume 10, Issue 2
Show Author Affiliations
Taisuke Ota, Osaka Univ. (Japan)
Hiroya Fukuyama, Olympus Corp. (Japan)
Yasuhige Ishihara, Olympus Corp. (Japan)
Hideo Tanaka, Kyoto Prefectural Univ. of Medicine (Japan)
Tetsuro Takamatsu, Kyoto Prefectural Univ. of Medicine (Japan)

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