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Journal of Biomedical Optics • Open Access

Photothermal responses of individual cells
Author(s): Dmitri Lapotko; Alexander I. Shnip; Ekaterina Luk'yanova

Paper Abstract

Photothermal (PT) responses of individual intact cells are studied with a thermal lens dual-laser scheme. A multiparameter model for analysis of PT responses as a function of cell size, structure, and optical properties is suggested and verified experimentally for living cells, red blood cells, lymphocytes, tumor cells (K 562), hepatocytes, and miocytes, by applying pulsed laser radiation at 532 nm for 10-ns duration. PT responses for noninvasive and damaging modes of laser-cell interaction are investigated. It is shown theoretically and experimentally that specific optical and structural features of cells influence the polarity, shape, front, and tail lengths of their PT responses. Common for different cells, features of PT responses are evaluated. It is found that in cells with a highly heterogeneous light-absorbing structure, the PT response of a whole cell differs from that of the local absorbing area. The model suggested allows us to interpret PT responses from single cells and to compare cells in terms of their diameter, degree of spatial heterogeneity of light absorbance, and laser-induced damage thresholds.

Paper Details

Date Published: 1 January 2005
PDF: 12 pages
J. Biomed. Opt. 10(1) 014006 doi: 10.1117/1.1854685
Published in: Journal of Biomedical Optics Volume 10, Issue 1
Show Author Affiliations
Dmitri Lapotko, A.V. Luikov Heat and Mass Transfer Institute (Belarus)
Alexander I. Shnip, A.V. Luikov Heat and Mass Transfer Institute (Belarus)
Ekaterina Luk'yanova, A.V. Luikov Heat and Mass Transfer Institute (Belarus)

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