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Journal of Biomedical Optics

Broadband reflectance measurements of light penetration, blood oxygenation, hemoglobin concentration, and drug concentration in human intraperitoneal tissues before and after photodynamic therapy
Author(s): Hsing-Wen Wang; Timothy C. Zhu; Mary P. Putt; Michael G. Solonenko; James M. Metz; Andreea Dimofte; Jeremy D. Miles; Douglas L. Fraker; Eli Glatstein; Stephen M. Hahn; Arjun G. Yodh
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Paper Abstract

We evaluate Photofrin-mediated photodynamic therapy (PDT) in a phase 2 clinical trial as an adjuvant to surgery to treat peritoneal carcinomatosis. We extract tissue optical [reduced scattering (µ's), absorption (µa), and attenuation coefficients (µeff)] and physiological [blood oxygen saturation (%StO2), total hemoglobin concentration (THC), and photosensitizer concentration (cPhotofrin)] properties in 12 patients using a diffuse reflectance instrument and algorithms based on the diffusion equation. Before PDT, in normal intraperitoneal tissues %StO2 and THC ranged between 32 to 100% and 19 to 263 µM, respectively; corresponding data from tumor tissues ranged between 11 to 44% and 61 to 224 µM. Tumor %StO2 is significantly lower than oxygenation of normal intraperitoneal tissues in the same patients. The mean (±standard error of mean) penetration depth (δ) in millimeters at 630 nm is 4.8(±0.6) for small bowel, 5.2 (±0.67) for large bowel, 3.39(±0.29) for peritoneum, 5.19(±1.4) for skin, 1.0(±0.1) for liver, and 3.02(±0.66) for tumor. cPhotofrin in micromolars is 4.9(±2.3) for small bowel, 4.8(±2.3) for large bowel, 3.0 (±1.0) for peritoneum, 2.5(±0.9) for skin, and 7.4(±2.8) for tumor. In all tissues examined, mean cPhotofrin tends to decrease after PDT, perhaps due to photobleaching. These results provide benchmark in-vivo tissue optical property data, and demonstrate the feasibility of in-situ measurements during clinical PDT treatments.

Paper Details

Date Published: 1 January 2005
PDF: 13 pages
J. Biomed. Opt. 10(1) 014004 doi: 10.1117/1.1854679
Published in: Journal of Biomedical Optics Volume 10, Issue 1
Show Author Affiliations
Hsing-Wen Wang, Univ. of Pennsylvania (United States)
Timothy C. Zhu, Univ. of Pennsylvania (United States)
Mary P. Putt, Univ. of Pennsylvania (United States)
Michael G. Solonenko, Univ. of Pennsylvania (United States)
James M. Metz, Univ. of Pennsylvania (United States)
Andreea Dimofte, Univ. of Pennsylvania (United States)
Jeremy D. Miles, Univ. of Pennsylvania (United States)
Douglas L. Fraker, Univ. of Pennsylvania (United States)
Eli Glatstein, Univ. of Pennsylvania (United States)
Stephen M. Hahn, Univ. of Pennsylvania (United States)
Arjun G. Yodh, Univ. of Pennsylvania (United States)

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