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Journal of Biomedical Optics

Intensity profiles of linearly polarized light backscattered from skin and tissue-like phantoms
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Paper Abstract

Anisotropy of mouse and human skin is investigated in vivo using polarized videoreflectometry. An incident beam (linearly polarized, wavelength 650 nm) is focused at the sample surface. Two types of tissuelike media are used as controls to verify the technique: isotropic delrin and highly anisotropic demineralized bone with a priori knowledge of preferential orientation of collagen fibers. Equi-intensity profiles of light, backscattered from the sample, are fitted with ellipses that appear to follow the orientation of the collagen fibers. The ratio of the ellipse semiaxes is well correlated with the ratio of reduced scattering coefficients obtained from radial intensity distributions. Variation of equi-intensity profiles with distance from the incident beam is analyzed for different initial polarization states of the light and the relative orientation of polarization filters for incident and backscattered light. For the anisotropic media (demineralized bone and human and mouse skin), a qualitative difference between intensity distributions for cross- and co-polarized orientations of the polarization analyzer is observed up to a distance of 1.5 to 2.5 mm from the entry point. The polarized videoreflectometry of the skin may be a useful tool to assess skin fibrosis resulting from radiation treatment.

Paper Details

Date Published: 1 January 2005
PDF: 9 pages
J. Biomed. Opt. 10(1) 014012 doi: 10.1117/1.1854677
Published in: Journal of Biomedical Optics Volume 10, Issue 1
Show Author Affiliations
Alexander P. Sviridov, Saratov State Univ. (Russia)
Victor V. Chernomordik, National Institutes of Health (United States)
Moinuddin Hassan, National Institutes of Health (United States)
Angelo Russo, National Institutes of Health (United States)
Alec B. Eidsath, National Institutes of Health (United States)
Paul D. Smith, National Institutes of Health (United States)
Amir H. Gandjbakhche, National Institutes of Health (United States)

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