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Journal of Biomedical Optics

Imaging ex vivo healthy and pathological human brain tissue with ultra-high-resolution optical coherence tomography
Author(s): Kostadinka K. Bizheva; Angelika Unterhuber; Boris M. Hermann; Boris Povazay; Harald Sattmann; Adolf Friedrich Fercher; Wolfgang Drexler; Matthias Preusser; Herbert Budka; Andreas Stingl; Tuan M. Le
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Paper Abstract

The ability of ultra-high-resolution optical coherence tomography (UHR OCT) to discriminate between healthy and pathological human brain tissue is examined by imaging ex vivo tissue morphology of various brain biopsies. Micrometer-scale OCT resolution (0.9×2 µm, axial×lateral) is achieved in biological tissue by interfacing a state-of-the-art Ti:Al2O3 laser (λc=800 nm, Δλ=260 nm, and Pout=120 mW exfiber) to a free-space OCT system utilizing dynamic focusing. UHR OCT images are acquired from both healthy brain tissue and various types of brain tumors including fibrous, athypical, and transitional meningioma and ganglioglioma. A comparison of the tomograms with standard hematoxylin and eosin (H&E) stained histological sections of the imaged biopsies demonstrates the ability of UHR OCT to visualize and identify morphological features such as microcalcifications (<20 µm), enlarged nuclei of tumor cells (~8 to 15 µm), small cysts, and blood vessels, which are characteristic of neuropathologies and normally absent in healthy brain tissue.

Paper Details

Date Published: 1 September 2007
PDF: 7 pages
J. Biomed. Opt. 10(1) 011006 doi: 10.1117/1.1851513
Published in: Journal of Biomedical Optics Volume 10, Issue 1
Show Author Affiliations
Kostadinka K. Bizheva, Univ. of Waterloo (Canada)
Angelika Unterhuber, Medical Univ. Vienna (Austria)
Boris M. Hermann, Medizinische Univ. Wien (Austria)
Boris Povazay, Medizinische Univ. Wien (Austria)
Harald Sattmann, Medizinische Univ. Wien (Austria)
Adolf Friedrich Fercher, Medizinische Univ. Wien (Austria)
Wolfgang Drexler, Medizinische Univ. Wien (Austria)
Matthias Preusser, Medizinische Univ. Wien (Austria)
Herbert Budka, Medizinische Univ. Wien (Austria)
Andreas Stingl, Femtolasers Produktions GmbH (Austria)
Tuan M. Le, Femtolasers Produktions GmbH (Austria)

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