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Journal of Biomedical Optics

Kerr-gated time-resolved Raman spectroscopy of equine cortical bone tissue
Author(s): Michael D. Morris; Pavel Matousek; Michael Towrie; Anthony W. Parker; Allen E. Goodship; Edward R.C. Draper
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Paper Abstract

Picosecond time-resolved Raman spectroscopy in equine cortical bone tissue is demonstrated. Using 400-nm pulsed laser excitation (1 ps at 1 kHz) it is shown that Kerr cell gating with a 4-ps window provides simultaneously time-resolved rejection of fluorescence and time-resolved Raman scatter enabling depth profiling through tissue. The Raman shifts are the same as those observed by conventional cw Raman spectroscopy using deep-red or near-infrared lasers. The time decay of Raman photons is shown to fit an inverse square root of time function, suggesting propagation by a diffusive mechanism. Using polystyrene behind a bone specimen, it is shown that the 400-nm laser light penetrates at least 0.31 mm below the surface of a fully mineralized bone tissue specimen and generates observable bone Raman scatter (approximately 415 to 430 nm) through most of this depth. These novel results demonstrate great promise for in vivo applications for studying diseased bone tissue, and ways to optimize the setup are discussed.

Paper Details

Date Published: 1 January 2005
PDF: 7 pages
J. Biomed. Opt. 10(1) 014014 doi: 10.1117/1.1827605
Published in: Journal of Biomedical Optics Volume 10, Issue 1
Show Author Affiliations
Michael D. Morris, Univ. of Michigan (United States)
Pavel Matousek, Rutherford Appleton Lab. (United Kingdom)
Michael Towrie, Rutherford Appleton Lab. (United Kingdom)
Anthony W. Parker, Rutherford Appleton Lab. (United Kingdom)
Allen E. Goodship, Royal Veterinary College (United Kingdom)
Edward R.C. Draper, Imperial College London (United Kingdom)

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