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Journal of Biomedical Optics

Three-dimensional optical tomographic brain imaging in small animals, part 2: Unilateral carotid occlusion
Author(s): Avraham Y. Bluestone; Mark Stewart; B. Lei; I. S. Kass; Joseph M. Lasker; Gassan S. Abdoulaev; Andreas H. Hielscher
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Paper Abstract

This is the second part of a two-part study that explores the feasibility of 3-D, volumetric brain imaging in small animals by optical tomographic techniques. In part 1, we demonstrated the ability to visualize global hemodynamic changes in the rat head in response to elevated levels of CO2 using a continuous-wave instrument and model-based iterative image reconstruction (MOBIIR) algorithm. Now we focus on lateralized, monohemispherically localized hemodynamic effects generated by unilateral common carotid artery (CCA) occlusion. This illustrates the capability of our optical tomographic system to localize and distinguish hemodynamic responses in different parts of the brain. Unilateral carotid occlusions are performed in ten rodents under two experimental conditions. In the first set of experiments the normal systemic blood pressure is lowered to 50 mmHg, and on unilateral carotid occlusion, we observe an ipsilateral monohemispheric global decrease in blood volume and oxygenation. This finding is consistent with the known physiologic response to cerebral ischemia. In a second set of experiments designed to observe the spatial-temporal dynamics of CCA occlusion at normotensive blood pressure, more complex phenomena are observed. We find three different types of responses, which can be categorized as compensation, overcompensation, and noncompensation.

Paper Details

Date Published: 1 September 2004
PDF: 11 pages
J. Biomed. Opt. 9(5) doi: 10.1117/1.1784472
Published in: Journal of Biomedical Optics Volume 9, Issue 5
Show Author Affiliations
Avraham Y. Bluestone, Columbia Univ. (United States)
Mark Stewart, SUNY/Downstate Medical Ctr. (United States)
B. Lei, SUNY/Downstate Medical Ctr (United States)
I. S. Kass, SUNY/Downstate Medical Ctr (United States)
Joseph M. Lasker, Columbia Univ. (United States)
Gassan S. Abdoulaev, Columbia Univ. (United States)
Andreas H. Hielscher, Columbia Univ. (United States)

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