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Journal of Biomedical Optics

Contaminant effect on cellular metabolic differential pressure curves
Author(s): Marziale Milani; Monica Ballerini; Lorenzo Ferraro; Matteo Zabeo; Massimo Barberis; Maria Cannone; Venera Faraone
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Paper Abstract

The possibility of a pressure monitoring system by differential pressure sensors to detect contaminant effects on cellular cultures metabolic activity is discussed using Saccharomyces cerevisiae, lymphocyte, and AHH1 cell cultures. Metabolic (aerobic and anaerobic) processes in cells are accompanied by CO2 production that induces changes in pressure values when cells are cultured in sealed vessels. These values are subsequently converted in voltage units and plotted pressure dynamics versus time. This procedure leads to a standard curve, typical of the cellular line, which characterizes cellular metabolism when all parameters are controlled, such as temperature and nutrients. Different phases appear in the S. cerevisiae differential pressure curve: an initial growth up to a maximum, followed by a decrement that leads to a typical "depression" (pressure values inside the test-tubes are lower than the initial one) after about 35 h from the beginning. The S. cerevisiae differential pressure curve is successfully used to test the effects of chemical (Amuchina®, trieline) and physical (UV radiation, blue light, magnetic fields) contaminants. The same technique is applied to lymphocytes and AHH1 cultures to investigate the effects generated by a 72-h exposure to a 50-Hz, 60-μT electromagnetic field. Lymphocyte samples, cultured in a PHA medium, grow less than control ones, but exhibit a greater metabolic activity: changes in the exposure system configuration influence neither sample growth differences nor metabolic response variations between control and irradiated samples, while all the other irradiation parameters remain constant. Control and irradiated lymphocyte samples, without PHA in culture medium, show the same behavior both during irradiation and metabolic test. AHH1 control and irradiated samples show no difference both in growth percentage during irradiation and in metabolic activity. Different cell cultures respond to the same stimulus in different manners.

Paper Details

Date Published: 1 September 2004
PDF: 15 pages
J. Biomed. Opt. 9(5) doi: 10.1117/1.1782591
Published in: Journal of Biomedical Optics Volume 9, Issue 5
Show Author Affiliations
Marziale Milani, Univ. degli Studi di Milano-Bicocca (Italy)
Monica Ballerini, Univ. degli Studi di Milano-Bicocca (Italy)
Lorenzo Ferraro, Univ. degli Studi di Milano-Bicocca (Italy)
Matteo Zabeo, Univ. degli Studi di Milano-Bicocca (Italy)
Massimo Barberis, Univ. degli Studi di Milano-Bicocca (Italy)
Maria Cannone, Multimedica Hospital (Italy)
Venera Faraone, Univ. degli Studi di Messina (Italy)

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