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Journal of Biomedical Optics

In vivo assessment of thermal damage in the liver using optical spectroscopy
Author(s): Clay R. Buttemere; Ravi S. Chari; Christopher Anderson; Kay Washington; Anita Mahadevan-Jansen; Wei-Chiang Lin
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Paper Abstract

Resection is not a viable treatment option for the majority of liver cancer patients. Alternatives to resection include thermotherapies such as radio-frequency ablation; however, these therapies lack adequate intraoperative feedback regarding the degree and margins of tissue thermal damage. In this proof of principle study, we test the ability of fluorescence and diffuse reflectance spectroscopy to assess local thermal damage in vivo. Spectra were acquired in vivo from healthy canine liver tissue undergoing radio-frequency ablation using a portable fiber-optic-based spectroscopic system. The major observed spectral alterations on thermal coagulation were a red shift in the fluorescence emission peak at 480 nm, a decrease in the overall fluorescence intensity, and an increase in the diffuse reflectance from 450 to 750 nm. Spectral changes were quantified and correlated to tissue histology. We found a good correlation between the proposed spectral correlates of thermal damage and histology. The results of this study suggest that fluorescence and diffuse reflectance spectroscopy show strong potential as tools to monitor liver tissue thermal damage intraoperatively.

Paper Details

Date Published: 1 September 2004
PDF: 10 pages
J. Biomed. Opt. 9(5) doi: 10.1117/1.1779627
Published in: Journal of Biomedical Optics Volume 9, Issue 5
Show Author Affiliations
Clay R. Buttemere, Vanderbilt Univ. (United States)
Ravi S. Chari, Vanderbilt Univ. (United States)
Christopher Anderson, Vanderbilt Univ. (United States)
Kay Washington, Vanderbilt Univ. (United States)
Anita Mahadevan-Jansen, Vanderbilt Univ. (United States)
Wei-Chiang Lin, Vanderbilt Univ. (United States)


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