Journal of Biomedical OpticsTwo-photon laser scanning fluorescence microscopy using photonic crystal fiber
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We report the application of a simple yet powerful modular pulse compression system based on photonic crystal fibers that improves on incumbent two-photon laser scanning fluorescence microscopy techniques. This system provides more than a sevenfold increase in fluorescence yield when compared with a commercial two-photon microscopy system. From this, we infer pulses of IR radiaton of less than 35 fs duration reaching the sample.