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Journal of Biomedical Optics

Confocal fluorescence microscope with dual-axis architecture and biaxial postobjective scanning
Author(s): Thomas D. Wang; Christopher H. Contag; Michael J. Mandella; Ning Y. Chan; Gordon S. Kino
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Paper Abstract

We present a novel confocal microscope that has dual-axis architecture and biaxial postobjective scanning for the collection of fluorescence images from biological specimens. This design uses two low-numerical-aperture lenses to achieve high axial resolution and long working distance, and the scanning mirror located distal to the lenses rotates along the orthogonal axes to produce arc-surface images over a large field of view (FOV). With fiber optic coupling, this microscope can potentially be scaled down to millimeter dimensions via microelectromechanical systems (MEMS) technology. We demonstrate a benchtop prototype with a spatial resolution ≤4.4 μm that collects fluorescence images with a high SNR and a good contrast ratio from specimens expressing GFP. Furthermore, the scanning mechanism produces only small differences in aberrations over the image FOV. These results demonstrate proof of concept of the dual-axis confocal architecture for in vivo molecular and cellular imaging.

Paper Details

Date Published: 1 July 2004
PDF: 8 pages
J. Biomed. Opt. 9(4) doi: 10.1117/1.1760760
Published in: Journal of Biomedical Optics Volume 9, Issue 4
Show Author Affiliations
Thomas D. Wang, Stanford Univ. (United States)
Christopher H. Contag, Stanford Univ. (United States)
Michael J. Mandella, ESPi (United States)
Ning Y. Chan, Optical Biopsy Technologies Inc. (United States)
Gordon S. Kino, Stanford Univ. (United States)

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