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Journal of Biomedical Optics • Open Access

Redox ratio of mitochondria as an indicator for the response of photodynamic therapy
Author(s): Zhihong Zhang; Dana Blessington; Hui Li; Theresa M. Busch; Jerry D. Glickson; Qingming Luo; Britton Chance; Gang Zheng

Paper Abstract

The effect of photodynamic therapy (PDT) treatment on the metabolic state of tumor mitochondria is investigated by imaging of tumor redox status. PDT is performed using the photosensitizer pyropheophorbide-2-deoxyglucosamide (Pyro-2DG), which utilizes the glucose import pathway. It is found that Pyro-2DG-induced PDT resulting in a highly oxidized state of tumor mitochondria. This is determined from the redox ratio changes derived from the intrinsic oxidized flavoprotein (Fp) and reduced pyridine nucleotide (PN) [i.e., reduced nicotinamide adenine dinucleotide (NADH)] fluorescence signals observed using a cryoimager. Thus, the redox ratio is a sensitive indicator for providing reliable and informative measurements of PDT-induced tissue damage. In the PDT treated region of the tumor, highly oxidized flavoprotein and diminishing NADH fluorescence is detected, suggesting that flavoprotein and NADH are oxidized by singlet oxygen produced in the photosensitization process.

Paper Details

Date Published: 1 July 2004
PDF: 7 pages
J. Biomed. Opt. 9(4) doi: 10.1117/1.1760759
Published in: Journal of Biomedical Optics Volume 9, Issue 4
Show Author Affiliations
Zhihong Zhang, Huazhong Univ. of Science and Technology (China)
Dana Blessington, Univ. of Pennsylvania (United States)
Hui Li, Univ. of Pennsylvania (United States)
Theresa M. Busch, Univ. of Pennsylvania (United States)
Jerry D. Glickson, Univ. of Pennsylvania (United States)
Qingming Luo, Huazhong Univ. of Science and Technology (China)
Britton Chance, Univ. of Pennsylvania (United States)
Gang Zheng, Univ. of Pennsylvania (United States)

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