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Journal of Biomedical Optics

Second-harmonic imaging of cornea after intrastromal femtosecond laser ablation
Author(s): Meng Han; Leander Zickler; Günter Giese; Matthias Walter; Frieder H. Loesel; Josef F. Bille
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Paper Abstract

Nonlinear laser scanning microscopy is widely used for noninvasive imaging in cell biology and tissue physiology. However, multiphoton fluorescence imaging of dense, transparent connective tissue (e.g., cornea) is challenging since sophisticated labeling or slicing is necessary. High-resolution, high-contrast second harmonic generation (SHG) imaging of corneal tissue based on the intrinsic structure of collagen is discussed. The three-dimensional corneal ultrastructure in depths up to hundreds of microns can be probed noninvasively, without any staining or mechanical slicing. As an important application of second harmonic imaging in ophthalmology, the modification of corneal ultrastructure using femtosecond laser intrastromal ablation is systematically investigated to evaluate next-generation refractive surgical approaches.

Paper Details

Date Published: 1 July 2004
PDF: 7 pages
J. Biomed. Opt. 9(4) doi: 10.1117/1.1756919
Published in: Journal of Biomedical Optics Volume 9, Issue 4
Show Author Affiliations
Meng Han, Ruprecht-Karls-Univ. Heidelberg (Germany)
Leander Zickler, Ultrafast Medical Lasers (Germany)
Günter Giese, Max-Planck-Institut für Medizinische Forschung (Germany)
Matthias Walter, Ruprecht-Karls-Univ. Heidelberg (Germany)
Frieder H. Loesel, 20/10 Perfect Vision GmbH (Germany)
Josef F. Bille, Ruprecht-Karls-Univ. Heidelberg (Germany)

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