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Journal of Biomedical Optics

Dual modality instrument for simultaneous optical coherence tomography imaging and fluorescence spectroscopy
Author(s): Jennifer Kehlet Barton; Francisco Guzman; Alexandre R. Tumlinson
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Paper Abstract

We develop a dual-modality device that combines the anatomical imaging capabilities of optical coherence tomography (OCT) with the functional capabilities of laser-induced fluorescence (LIF) spectroscopy. OCT provides cross-sectional images of tissue structure to a depth of up to 2 mm with approximately 10-μm resolution. LIF spectroscopy provides histochemical information in the form of emission spectra from a given tissue location. The OCT subsystem utilizes a superluminescent diode with a center wavelength of 1300 nm, whereas a helium cadmium laser provides the LIF excitation source at wavelengths of 325 and 442 nm. Preliminary data are obtained on eight postmortem aorta samples, each 10 mm in length. OCT images and LIF spectra give complementary information from normal and atherosclerotic portions of aorta wall. OCT images show structures such as intima, media, internal elastic lamina, and fibrotic regions. Emission spectra ratios of 520/490 (325-nm excitation) and 595/635 (442-nm excitation) could be used to identify normal and plaque regions with 97 and 91% correct classification rates, respectively. With miniaturization of the delivery probe and improvements in system speed, this dual-modality device could provide a valuable tool for identification and characterization of atherosclerotic plaques.

Paper Details

Date Published: 1 May 2004
PDF: 6 pages
J. Biomed. Opt. 9(3) doi: 10.1117/1.1695564
Published in: Journal of Biomedical Optics Volume 9, Issue 3
Show Author Affiliations
Jennifer Kehlet Barton, Univ. of Arizona (United States)
Francisco Guzman, Optical Sciences Ctr./Univ. of Arizona (United States)
Alexandre R. Tumlinson, Univ. of Arizona (United States)

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