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Optical Engineering

Formation of a doughnut laser beam for super-resolving microscopy using a phase spatial light modulator
Author(s): Takeshi Watanabe; Yasuhiro Igasaki; Norihiro Fukuchi; Makoto Sakai; Shun-ichi Ishiuchi; Masaaki Fujii; Takashige Omatsu; Kimihisa Yamamoto; Yoshinori Iketaki
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Paper Abstract

The formation of a doughnut-shaped laser beam is presented. To generate the beam, we use an optically addressed parallel-aligned nematic liquid-crystal phase spatial light modulator (PAL-SLM), and observe the shape of the focused beam. By using a compensating technique for wave aberration, the beam has a symmetric doughnut shape with a hole size of 1 μmφ on the focal plane. The experimental result shows that the generated beam can be expected to be applicable to super-resolving microscopy based on the fluorescence depletion process.

Paper Details

Date Published: 1 May 2004
PDF: 8 pages
Opt. Eng. 43(5) doi: 10.1117/1.1690767
Published in: Optical Engineering Volume 43, Issue 5
Show Author Affiliations
Takeshi Watanabe, Tokyo Institute of Technology (Japan)
Yasuhiro Igasaki, Hamamatsu Photonics KK (Japan)
Norihiro Fukuchi, Hamamatsu Photonics KK (Japan)
Makoto Sakai, Tokyo Institute of Technology (Japan)
Shun-ichi Ishiuchi, Tokyo Institute of Technology (Japan)
Masaaki Fujii, Tokyo Institute of Technology (Japan)
Takashige Omatsu, Chiba Univ. (Japan)
Kimihisa Yamamoto, Keio Univ. (Japan)
Yoshinori Iketaki, Olympus Optical Co., Ltd. (Japan)


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