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Journal of Biomedical Optics

Imaging wound healing using optical coherence tomography and multiphoton microscopy in an in vitro skin-equivalent tissue model
Author(s): Alvin T. Yeh; Bunsho Kao; Woong Gyu Jung; Zhongping Chen; John Stuart Nelson; Bruce J. Tromberg
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Paper Abstract

Laser thermal injury and subsequent wound healing in organotypic, skin-equivalent tissue models were monitored using optical coherence tomography (OCT), multiphoton microscopy (MPM), and histopathology. The in vitro skin-equivalent raft tissue model was composed of dermis with type I collagen and fibroblast cells and epidermis of differentiated keratinocytes. Noninvasive optical imaging techniques were used for time-dependent, serial measurements of matrix destruction and reconstruction and compared with histopathology. The region of laser thermal injury was clearly delineated in OCT images by low signal intensity. High resolution MPM imaging using second-harmonic generation revealed alterations in collagen microstructure organization with subsequent matrix reconstruction. Fibroblast cell migration in response to injury was monitored by MPM using two-photon excited fluorescence. This study illustrates the complementary features of linear and nonlinear light–tissue interaction in intrinsic signal optical imaging and their use for noninvasive, serial monitoring of wound healing processes in biological tissues.

Paper Details

Date Published: 1 March 2004
PDF: 6 pages
J. Biomed. Opt. 9(2) doi: 10.1117/1.1648646
Published in: Journal of Biomedical Optics Volume 9, Issue 2
Show Author Affiliations
Alvin T. Yeh, Univ. of California/Irvine (United States)
Bunsho Kao, Univ. of California/Irvine (United States)
Woong Gyu Jung, Univ. of California/Irvine (United States)
Zhongping Chen, Univ. of California/Irvine (United States)
John Stuart Nelson, Univ. of California/Irvine (United States)
Bruce J. Tromberg, Univ. of California/Irvine (United States)


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