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Journal of Biomedical Optics

Optical imaging of Renilla luciferase, synthetic Renilla luciferase, and firefly luciferase reporter gene expression in living mice
Author(s): S. Bhaumik; X. Z. Lewis; Sanjiv Gambhir
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Paper Abstract

We have recently demonstrated that Renilla luciferase (Rluc) is a promising bioluminescence reporter gene that can be used for noninvasive optical imaging of reporter gene expression in living mice, with the aid of a cooled charged couple device (CCD) camera. In the current study, we explore the expression of a novel synthetic Renilla luciferase reporter gene (hRluc) in living mice, which has previously been reported to be a more sensitive reporter than native Rluc in mammalian cells. We explore the strategies of simultaneous imaging of both Renilla luciferase enzyme (RL) and synthetic Renilla luciferase enzyme (hRL):coelenterazine (substrate for RL/hRL) in the same living mouse. We also demonstrate that hRL:coelenterazine can yield a higher signal when compared to Firefly luciferase enzyme (FL): D-Luciferin, both in cell culture studies and when imaged from cells at the surface and from lungs of living mice. These studies demonstrate that hRluc should be a useful primary reporter gene with high sensitivity when used alone or in conjunction with other bioluminescence reporter genes for imaging in living rodents.

Paper Details

Date Published: 1 May 2004
PDF: 9 pages
J. Biomed. Opt. 9(3) doi: 10.1117/1.1647546
Published in: Journal of Biomedical Optics Volume 9, Issue 3
Show Author Affiliations
S. Bhaumik, Univ. of California/Los Angeles (United States)
X. Z. Lewis, Univ. of California/Los Angeles (United States)
Sanjiv Gambhir, Univ. of California/Los Angeles (United States)

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