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Journal of Biomedical Optics

Digital image capture and quantification of subtle lens opacities in rodents
Author(s): T. M. Seeberger; Y. Matsumoto; A. Alizadeh; Paul G. Fitzgerald; John I. Clark
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Paper Abstract

A rapid, sensitive, and cost-effective method is reported for the subjective and objective documentation of subtle opacities in lenses of unanesthetized transgenic mice or selenite-injected rat pups as models for cataract formation. Animal eyes were dilated with eye drops and the animal was positioned in front of a Nikon FS2 photo slit lamp. Slit-lamp observations were recorded using a Canon Optura Pi digital video recorder. High-quality images of opacifying lenses were captured from the video and quantified using densitometry at progressive stages of opacification. In mice, targeted genomic deletion of the proteins CP49 (a lens-specific filament) or Six5 (a model for myotonic dystrophy) resulted in subtle cataracts that were easily recorded and quantified using this instrumentation. In rats, the early progressive changes leading to a dense nuclear opacity caused by selenite injection were easily documented using this instrumentation. Low-cost components combined with a conventional slit-lamp ophthalmoscope were used to capture high-quality images of selected stages of cataract formation for quantitative analysis using commercial software.

Paper Details

Date Published: 1 January 2004
PDF: 5 pages
J. Biomed. Opt. 9(1) doi: 10.1117/1.1630034
Published in: Journal of Biomedical Optics Volume 9, Issue 1
Show Author Affiliations
T. M. Seeberger, Univ. of Washington (United States)
Y. Matsumoto, Dokkyo Univ. School of Medicine (Japan)
A. Alizadeh, Univ. of California (United States)
Paul G. Fitzgerald, Univ. of California/Davis (United States)
John I. Clark, Univ. of Washington (United States)


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