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Journal of Biomedical Optics

Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods
Author(s): Enrico Gratton; Sophie Breusegem; Jason D. B. Sutin; Qiaoaio Ruan; Nicholas P. Barry
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Paper Abstract

Fluorescence lifetime images are obtained with the laser scanning microscope using two methods: the time-correlated single-photon counting method and the frequency-domain method. In the same microscope system, we implement both methods. We perform a comparison of the performance of the two approaches in terms of signal-to-noise ratio (SNR) and the speed of data acquisition. While in our practical implementation the time-correlated single-photon counting technique provides a better SNR for low-intensity images, the frequency-domain method is faster and provides less distortion for bright samples.

Paper Details

Date Published: 1 July 2003
PDF: 10 pages
J. Biomed. Opt. 8(3) doi: 10.1117/1.1586704
Published in: Journal of Biomedical Optics Volume 8, Issue 3
Show Author Affiliations
Enrico Gratton, Univ. of Illinois/Urbana-Champaign (United States)
Sophie Breusegem, Univ. of Illinois/Urbana-Champaign (United States)
Jason D. B. Sutin, Univ. of Illinois/Urbana-Champaign (United States)
Qiaoaio Ruan, Univ. of Illinois/Urbana-Champaign (United States)
Nicholas P. Barry, Univ. of Illinois/Urbana-Champaign (United States)


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