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Journal of Biomedical Optics

Mechanisms of membrane potential sensing with second-harmonic generation microscopy
Author(s): Thomas Pons; Laurent Moreaux; Olivier Mongin; Mireille H. Blanchard-Desce; Jerome Mertz
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Paper Abstract

We characterize the transmembrane voltage response of a novel second-harmonic generation (SHG) marker using a screening protocol with giant unilamellar vesicles. Two mechanisms are found to contribute to the voltage response: (1) an electro-optic-induced alteration of the molecular hyperpolarizability and (2) an electric-field-induced alteration of the degree of molecular alignment. We quantify the relative weights and of these contributions and provide an upper limit to their response time, which is found to be submillisecond. The identification of two voltage response mechanisms leads to new strategies for the molecular design of membrane potential markers.

Paper Details

Date Published: 1 July 2003
PDF: 4 pages
J. Biomed. Opt. 8(3) doi: 10.1117/1.1581871
Published in: Journal of Biomedical Optics Volume 8, Issue 3
Show Author Affiliations
Thomas Pons, Ecole Superieure de Physique et de Chimie Indust. (France)
Laurent Moreaux, Ctr. National de la Recherche Scientifique (France)
Olivier Mongin, Univ. de Rennes I (France)
Mireille H. Blanchard-Desce, Univ. de Rennes I (France)
Jerome Mertz, Ecole Superieure de Physique et de Chimie Indust. (France)


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