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Journal of Biomedical Optics

Quantitative imaging of protein–protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera
Author(s): Ramanujan Venkata Krishnan; Atsushi Masuda; Victoria Frohlich E. Centonze; Brian A. Herman
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Paper Abstract

Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein–protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak camera is described. An example of a typical application of the system is provided in which the fluorescence resonance energy transfer between a donor-acceptor pair of fluorescent proteins within a cellular specimen is measured.

Paper Details

Date Published: 1 July 2003
PDF: 6 pages
J. Biomed. Opt. 8(3) doi: 10.1117/1.1577574
Published in: Journal of Biomedical Optics Volume 8, Issue 3
Show Author Affiliations
Ramanujan Venkata Krishnan, Univ. of Texas (United States)
Atsushi Masuda, Univ. of Texas (United States)
Victoria Frohlich E. Centonze, Univ. of Texas (United States)
Brian A. Herman, Univ. of Texas (United States)


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