Share Email Print

Journal of Biomedical Optics

Correlation of in vivo photosensitizer fluorescence and photodynamic-therapy-induced depth of necrosis in a murine tumor model
Author(s): Rex Cheung; Michael Solonenko; Theresa M. Busch; Fabio Del Piero; M. P. Putt; Stephen M. Hahn; Arjun G. Yodh
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We compared light-induced fluorescence (LIF) to nominal injected drug dose for predicting the depth of necrosis response to photodynamic therapy (PDT) in a murine tumor model. Mice were implanted with radiation-induced fibrosarcoma (RIF) and were injected with 0, 5, or 10 mg/kg Photofrin. 630-nm light (30 J/cm2, 75 mW/cm2) was delivered to the tumor after 24 hours. Fluorescence emission (λexcitation = 545 nm, λemission = 628 nm) from the tumor was measured. The LIF data had less scatter than injected drug dose, and was found to be at least as good as an injected drug dose for predicting the depth of necrosis after PDT. Our observations provide further evidence that fluorescence spectroscopy can be used to quantify tissue photosensitizer uptake and to predict PDT tissue damage.

Paper Details

Date Published: 1 April 2003
PDF: 5 pages
J. Biomed. Opt. 8(2) doi: 10.1117/1.1560011
Published in: Journal of Biomedical Optics Volume 8, Issue 2
Show Author Affiliations
Rex Cheung, Univ. of Pennsylvania (United States)
Michael Solonenko, Univ. of Pennsylvania (United States)
Theresa M. Busch, Univ. of Pennsylvania (United States)
Fabio Del Piero, Univ. of Pennsylvania (United States)
M. P. Putt, Univ. of Pennsylvania (United States)
Stephen M. Hahn, Univ. of Pennsylvania (United States)
Arjun G. Yodh, Univ. of Pennsylvania (United States)

© SPIE. Terms of Use
Back to Top