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Journal of Biomedical Optics

Reflectance-based determination of optical properties in highly attenuating tissue
Author(s): T. Joshua Pfefer; L. Stephanie Matchette; Carrie L. Bennett; Jessica A. Gall; Joy A. Wilke; Anthony Joseph Durkin; Marwood N. Ediger
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Paper Abstract

Accurate data on in vivo tissue optical properties in the ultraviolet A (UVA) to visible (VIS) range are needed to elucidate light propagation effects and to aid in identifying safe exposure limits for biomedical optical spectroscopy. We have performed a preliminary study toward the development of a diffuse reflectance system with maximum fiber separation distance of less than 2.5 mm. The ultimate objective is to perform endoscopic measurement of optical properties in the UVA to VIS. Optical property sets with uniformly and randomly distributed values were developed within the range of interest: absorption coefficients from 1 to 25 cm–1 and reduced scattering coefficients from 5 to 25 cm–1. Reflectance datasets were generated by direct measurement of Intralipid-dye tissue phantoms at λ = 675 nm and Monte Carlo simulation of light propagation. Multivariate calibration models were generated using feed-forward artificial neural network or partial least squares algorithms. Models were calibrated and evaluated using simulated or measured reflectance datasets. The most accurate models developed—those based on a neural network and uniform optical property intervals—were able to determine absorption and reduced scattering coefficients with root mean square errors of ±2 and ±3 cm–1, respectively. Measurements of ex vivo bovine liver at 543 and 633 nm were within 5 to 30% of values reported in the literature. While our technique for determination of optical properties appears feasible and moderately accurate, enhanced accuracy may be achieved through modification of the experimental system and processing algorithms.

Paper Details

Date Published: 1 April 2003
PDF: 10 pages
J. Biomed. Opt. 8(2) doi: 10.1117/1.1559487
Published in: Journal of Biomedical Optics Volume 8, Issue 2
Show Author Affiliations
T. Joshua Pfefer, U.S. Food and Drug Administration (United States)
L. Stephanie Matchette, U.S. Food and Drug Administration (United States)
Carrie L. Bennett, Marquette Univ. (United States)
Jessica A. Gall, Marquette Univ. (United States)
Joy A. Wilke, Marquette Univ. (United States)
Anthony Joseph Durkin, Beckman Laser Institute and Medical Clinic (United States)
Marwood N. Ediger, U.S. Food and Drug Administration (United States)

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