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Journal of Biomedical Optics

Light scattering from cervical cells throughout neoplastic progression: influence of nuclear morphology, DNA content, and chromatin texture
Author(s): Rebekah A. Drezek; Martial Guillaud; Thomas G. Collier; Iouri Boiko; Anais Malpica; Calum E. MacAulay; Michele Follen; Rebecca R. Richards-Kortum
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Paper Abstract

A number of noninvasive fiber optic optical technologies are under development for real-time diagnosis of neoplasia. We investigate how the light scattering properties of cervical cells are affected by changes in nuclear morphology, DNA content, and chromatin texture, which occur during neoplastic progression. We used a Cyto-Savant computer-assisted image analysis system to acquire quantitative nuclear features measurements from 122 Feulgen-thionin-stained histopathologic sections of cervical tissue. A subset of the measured nuclear features was incorporated into a finite-difference time-domain (FDTD) model of cellular light scattering. The magnitude and angular distribution of scattered light was calculated for cervical cells as a function of pathologic grade. The nuclear atypia strongly affected light scattering properties. The increased size and elevated DNA content of nuclei in high-grade lesions caused the most significant changes in scattering intensity. The spatial dimensions of chromatin texture features and the amplitude of refractive index fluctuations within the nucleus impacted both the angular distribution of scattering angles and the total amount of scattered light. Cellular scattering is sensitive to changes in nuclear morphology that accompany neoplastic progression. Understanding the quantitative relationships between nuclear features and scattering properties will aid in the development of noninvasive optical technologies for detection of precancerous conditions.

Paper Details

Date Published: 1 January 2003
PDF: 10 pages
J. Biomed. Opt. 8(1) doi: 10.1117/1.1528950
Published in: Journal of Biomedical Optics Volume 8, Issue 1
Show Author Affiliations
Rebekah A. Drezek, Rice Univ. (United States)
Martial Guillaud, British Columbia Cancer Agency (Canada)
Thomas G. Collier, Univ. of Texas/Austin (United States)
Iouri Boiko, Univ. of Texas/Houston (United States)
Anais Malpica, Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Calum E. MacAulay, BC Cancer Agency (Canada)
Michele Follen, Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Rebecca R. Richards-Kortum, Univ. of Texas/Austin (United States)

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