Share Email Print

Journal of Biomedical Optics

Diagnostic potential of FTIR microspectroscopy and advanced computational methods in colon cancer patients
Author(s): Shmuel Argov; Jagannathan Ramesh; Ahmad Salman; Igor Sinelnikov; Jed Goldstein; Hugo Guterman; Shaul Mordechai
Format Member Price Non-Member Price
PDF $20.00 $25.00

Paper Abstract

Colon cancer is the third leading class of cancer causing increased mortality in developed countries. A polyp is one type of lesion observed in a majority of colon cancer patients. Here, we report a microscopic Fourier transform infrared (FTIR) study of normal, adenomatous polyp and malignant cells from biopsies of 24 patients. The goal of our study was to differentiate an adenomatous polyp from a malignant cell using FTIR microspectroscopy and artificial neural network (ANN) analysis. FTIR spectra and biological markers such as phosphate, RNA/DNA derived from spectra, were useful in identifying normal cells from abnormal ones that consisted of adenomatous polyp and malignant cells. However, the biological markers failed to differentiate between adenomatous polyp and malignant cases. By employing a combination of wavelet features and an ANN based classifier, we were able to classify the different cells as normal, adenomatous polyp and cancerous in a given tissue sample. The percentage of success of classification was 89%, 81%, and 83% for normal, adenomatous polyp, and malignant cells, respectively. A comparison of the method proposed with the pathological method is also discussed.

Paper Details

Date Published: 1 April 2002
PDF: 7 pages
J. Biomed. Opt. 7(2) doi: 10.1117/1.1463051
Published in: Journal of Biomedical Optics Volume 7, Issue 2
Show Author Affiliations
Shmuel Argov, Soroka Univ. Medical Ctr. (Israel)
Jagannathan Ramesh, Ben-Gurion Univ. of the Negev (Israel)
Ahmad Salman, Ben-Gurion Univ. of the Negev (Israel)
Igor Sinelnikov, Soroka Univ. Medical Ctr. (Israel)
Jed Goldstein, Ben-Gurion Univ. of the Negev (Israel)
Hugo Guterman, Ben-Gurion Univ. of the Negev (Israel)
Shaul Mordechai, Ben-Gurion Univ. of the Negev (Israel)

© SPIE. Terms of Use
Back to Top