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Journal of Biomedical Optics

Laser activated fluorescence measurements and morphological features: an in vivo study of clearance time of FITC tagged cell markers
Author(s): Israel Gannot; Gallya Gannot; A. Garashi; Amir H. Gandjbakhche; A. Buchner; Yona Keisari
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Paper Abstract

Fourteen BALB/c mice were divided into two groups. One group served as the control and the second group was injected with a squamous cell carcinoma cell line to the tongue. After tumor development (1–4 weeks), mice were injected with a FITC conjugated CD3 marker to their tongues. Immediately after the marker injection, the clearance of the marker was measured using a laser spectroscopy system. The markers were excited by an argon laser at 488 nm and the fluorescence signal was measured as a function of time. A biopsy was taken from every mouse after the procedure and the excised tissue was histologically evaluated. Analysis of clearance times revealed a second order exponential decay for both groups with a slower pace of signal clearance for the sick mice.

Paper Details

Date Published: 1 January 2002
PDF: 6 pages
J. Biomed. Opt. 7(1) doi: 10.1117/1.1427913
Published in: Journal of Biomedical Optics Volume 7, Issue 1
Show Author Affiliations
Israel Gannot, Tel-Aviv Univ. (Israel)
Gallya Gannot, Tel-Aviv Univ. (Israel)
A. Garashi, Tel-Aviv Univ. (Israel)
Amir H. Gandjbakhche, National Institutes of Health (Israel)
A. Buchner, Tel-Aviv Univ. (Israel)
Yona Keisari, Tel-Aviv Univ. (Israel)

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