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Journal of Biomedical Optics

Understanding the contributions of NADH and collagen to cervical tissue fluorescence spectra: modeling, measurements, and implications
Author(s): Rebekah A. Drezek; Konstantin V. Sokolov; Urs Utzinger; Iouri Boiko; Anais Malpica; Michele Follen; Rebecca R. Richards-Kortum
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Paper Abstract

Objective: At 380 nm excitation, cervical tissue fluorescence spectra demonstrate characteristic changes with both patient age and the presence of dysplasia. A Monte Carlo model was developed in order to quantitatively examine how intrinsic NADH and collagen fluorescence, in combination with tissue scattering and absorption properties, yield measured tissue spectra. Methods: Excitation-emission matrices were measured for live cervical cells and collagen gel phantoms. Fluorescence microscopy of fresh tissue sections was performed to obtain the location and density of fluorophores as a function of patient age and the presence of dysplasia. A Monte Carlo model was developed which incorporated measurements of fluorophore line shapes and spatial distributions. Results: Modeled spectra were consistent with clinical measurements and indicate that an increase in NADH fluorescence and decrease in collagen fluorescence create clinically observed differences between normal and dysplastic tissue spectra. Model predictions were most sensitive to patient age and epithelial thickness. Conclusions: Monte Carlo techniques provide an important means to investigate the combined contributions of multiple fluorophores to measured emission spectra. The approach will prove increasingly valuable as a more sophisticated understanding of in vivo optical properties is developed.

Paper Details

Date Published: 1 October 2001
PDF: 12 pages
J. Biomed. Opt. 6(4) doi: 10.1117/1.1413209
Published in: Journal of Biomedical Optics Volume 6, Issue 4
Show Author Affiliations
Rebekah A. Drezek, Univ. of Texas/Austin (United States)
Konstantin V. Sokolov, Univ. of Texas/Austin (United States)
Urs Utzinger, Univ. of Texas/Austin (United States)
Iouri Boiko, Univ. of Texas/Houston (United States)
Anais Malpica, Univ. of Texas/Houston (United States)
Michele Follen, Univ. of Texas/Houston (United States)
Rebecca R. Richards-Kortum, Univ. of Texas/Austin (United States)

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