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Spie Press Book

Live-Cell Optical Microscopy with Limited Light Doses
Author(s): Herbert Schneckenburger; Verena Richter; Michael Wagner
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Book Description

Live-cell microscopy is a rapidly growing field for the detection of cell or tissue architecture and for cell metabolism. This Spotlight examines the early detection of diseases and cellular responses to pharmaceutical agents with 3-D systems and non-phototoxic light doses. Techniques discussed in this eBook include 3-D microscopy, super-resolution microscopy, membrane imaging, long-exposure imaging, and spectral imaging.

Book Details

Date Published: 17 August 2018
Pages: 44
ISBN: 9781510622593
Volume: SL42

Table of Contents
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1 Introduction
1.1 Challenge 1: 3-D microscopy
1.2 Challenge 2: super-resolution microscopy
1.3 Challenge 3: membrane imaging
1.4 Challenge 4: long time exposure
1.5 Further challenges

2 Methods and Applications
2.1 Cell cultivation
2.2 Wide-field microscopy
2.3 Confocal laser scanning microscopy
2.4 Light-sheet fluorescence microscopy
2.5 Structured illumination microscopy
2.6 Axial tomography
2.7 Total internal reflection fluorescence microscopy
2.8 Spectral imaging, FLIM, and FRET

3 Concluding Remarks

Preface

The study of living organisms with light has been a challenge for several decades. Modern methods of microscopy are appropriate tools for this purpose, but their benefits should be harnessed while avoiding their harmful effects. This Spotlight examines the early detection of diseases and cellular responses to pharmaceutical agents with 3-D systems and non-phototoxic light doses. Techniques discussed in this eBook include 3-D microscopy, super-resolution microscopy, membrane imaging, long-exposure imaging, and spectral imaging.

Herbert Schneckenburger
Verena Richter
Michael Wagner
July 2018


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