The Moscone Center
San Francisco, California, United States
13 - 18 February 2016
Conference 9712
Multiphoton Microscopy in the Biomedical Sciences XVI
Sunday - Tuesday 14 - 16 February 2016
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Sunday 14 February Show All Abstracts
Opening Remarks
Sunday 14 February 2016
8:15 AM - 8:30 AM
Location: Room 2014 (West Level 2)

Conference Chair: Ammasi Periasamy, Univ. of Virginia (United States)
Session 1:
Keynote Session
Sunday 14 February 2016
8:30 AM - 10:00 AM
Location: Room 2014 (West Level 2)
Correlated phosphorescence and fluorescence lifetime imaging for cell metabolism (Keynote Presentation)
Paper 9712-1
Time: 8:30 AM - 9:00 AM
Author(s): Angelika C. Rueck, Jasmin Breymayer, P. Schäfer, Bjorn von Einem, Christine A. F. von Arnim, Sviatlana Kalinina, Univ. Ulm (Germany)
Show Abstract
Depth-resolved incoherent and coherent wide-field high-content imaging (Keynote Presentation)
Paper 9712-2
Time: 9:00 AM - 9:30 AM
Author(s): Peter T. C. So, Massachusetts Institute of Technology (United States)
Show Abstract
Biomedical applications of SRS microscopy (Keynote Presentation)
Paper 9712-3
Time: 9:30 AM - 10:00 AM
Author(s): Xiaoliang S. Xie, Harvard Univ. (United States)
Show Abstract
Coffee Break 10:00 AM - 10:30 AM
Session 2:
Biomedical Applications of Coherent Raman I
Sunday 14 February 2016
10:30 AM - 11:55 AM
Location: Room 2014 (West Level 2)
Label-free vibrational stark imaging of neuronal membrane potential (Invited Paper)
Paper 9712-4
Time: 10:30 AM - 10:50 AM
Author(s): Ji-Xin Cheng, Purdue Univ. (United States)
Show Abstract
Phenotype classification of single cells using coherent Raman microscopy and RNA sequencing
Paper 9712-5
Time: 10:50 AM - 11:05 AM
Author(s): Aaron M. Streets, Chen Cao, Xiannian Zhang, Yanyi Huang, Peking Univ. (China)
Show Abstract
Biological application of stimulated Raman scattering microscopy in modeling lipid dynamics (Invited Paper)
Paper 9712-6
Time: 11:05 AM - 11:25 AM
Author(s): Yong Yu, Baylor College of Medicine (United States); Dan Fu, Univ. of Washington (United States); Harrison Liu, Univ. of California, San Francisco (United States); Xiaoliang S. Xie, Harvard Univ. (United States); Bo Huang, Univ. of California, San Francisco (United States); Meng Wang, Baylor College of Medicine (United States)
Show Abstract
In vivo lipid saturation study of C. elegans using quantitative broadband coherent anti-stokes Raman imaging
Paper 9712-7
Time: 11:25 AM - 11:40 AM
Author(s): Bradley Littleton, Thomas Kavanagh, Yu Nie, Vincenzo Abbate, Peter Hylands, Stephen Sturzenbaum, David R. Richards, King's College London (United Kingdom)
Show Abstract
Limitations and solutions for polarization resolved in-depth nonlinear vibrational imaging in biological tissues
Paper 9712-8
Time: 11:40 AM - 11:55 AM
Author(s): Sophie Brasselet, Hilton B. de Aguiar, Paulina Gasecka, Julien Duboisset, Hervé Rigneault, Institut Fresnel (France)
Show Abstract
Lunch/Exhibition Break 11:55 AM - 1:25 PM
Session 3:
Biomedical Applications of Coherent Raman II
Sunday 14 February 2016
1:25 PM - 3:05 PM
Location: Room 2014 (West Level 2)
Broadband CARS: instrumentation, quantitation, and application (Invited Paper)
Paper 9712-9
Time: 1:25 PM - 1:45 PM
Author(s): Marcus T. Cicerone, Charles H. Camp, National Institute of Standards and Technology (United States)
Show Abstract
Intraoperative stimulated Raman scattering microscopy for guidance during brain tumor surgery
Paper 9712-10
Time: 1:45 PM - 2:00 PM
Author(s): Spencer Lewis, Univ. of Michigan Medical School (United States); Mia Garrard, Univ. of Michigan Health System (United States); Sunney S. Xie, Harvard Univ. (United States); Daniel A. Orringer, Univ. of Michigan Health System (United States)
Show Abstract
Histological reconstruction, guided micro-dissection, and sequencing of cancer tissue using stimulated Raman scattering (SRS) microscopy
Paper 9712-11
Time: 2:00 PM - 2:15 PM
Author(s): Tao Chen, Chen Cao, Biodynamic Optical Imaging Ctr. (China); Jianyun Zhang, Peking Univ. School of Stomatology (China); Yanyi Huang, Biodynamic Optical Imaging Ctr. (China); Tiejun Li, Peking Univ. School of Stomatology (China)
Show Abstract
Stimulated Raman scattering imaging of metabolic activities of mammalian brain tissue (Invited Paper)
Paper 9712-12
Time: 2:15 PM - 2:35 PM
Author(s): Fanghao Hu, Lu Wei, Michael R. Lamprecht, Barclay Morisson, Wei Min, Columbia Univ. (United States)
Show Abstract
Hyperspectral stimulated Raman scattering and multiphoton imaging for label-free colonic diseases diagnosis and characterization
Paper 9712-13
Time: 2:35 PM - 2:50 PM
Author(s): Zi Wang, Wei Zheng, Jian Lin, Zhiwei Huang, National Univ. of Singapore (Singapore)
Show Abstract
Biomedical lipid imaging in zebrafish with stimulated Raman scattering microscopy
Paper 9712-14
Time: 2:50 PM - 3:05 PM
Author(s): Miriam J. Moester, Marjo J. den Broeder, Freek Ariese, Juliette Legler, Johannes F. de Boer, Vrije Univ. Amsterdam (Netherlands)
Show Abstract
Coffee Break 3:05 PM - 3:35 PM
Session 4:
Coherent Raman Technical Development
Sunday 14 February 2016
3:35 PM - 5:10 PM
Location: Room 2014 (West Level 2)
  • Session Chair:
  • Marcus T. Cicerone, National Institute of Standards and Technology (United States)
Surface-sensitive coherent Raman scattering microscopy with total internal reflection illumination (Invited Paper)
Paper 9712-15
Time: 3:35 PM - 3:55 PM
Author(s): Eric O. Potma, Alex Fast, Univ. of California, Irvine (United States); Christropher D. Syme, Univ. of Glasgow (United Kingdom)
Show Abstract
Dispersion-based stimulated Raman scattering spectroscopy, holography, and optical coherence tomography
Paper 9712-16
Time: 3:55 PM - 4:10 PM
Author(s): Francisco E. Robles, Martin C. Fischer, Warren S. Warren, Duke Univ. (United States)
Show Abstract
Polarization modulated background-free hyperspectral stimulated Raman scattering microscopy
Paper 9712-17
Time: 4:10 PM - 4:25 PM
Author(s): Marie-Andrée Houle, Institut National de la Recherche Scientifique (Canada); Marco Andreana, National Research Council Canada (Canada), Univ. of Ottawa (Canada); Andrew Ridsdale, Douglas J. Moffatt, Rune Lausten, National Research Council Canada (Canada); François Légaré, Institut National de la Recherche Scientifique (Canada); Albert Stolow, National Research Council Canada (Canada), Univ. of Ottawa (Canada)
Show Abstract
Resonant artefacts in broadband cars microspectroscopy
Paper 9712-18
Time: 4:25 PM - 4:40 PM
Author(s): Bradley Littleton, Thomas Kavanagh, David R. Richards, King's College London (United Kingdom)
Show Abstract
Electric field mapping and dosimetry by means of spontaneous and coherent Raman microspectroscopy
Paper 9712-19
Time: 4:40 PM - 4:55 PM
Author(s): Erwan Capitaine, Christophe Louot, Claire Lefort, Dominique Pagnoux, Vincent Couderc, Philippe Leproux, XLIM Institut de Recherche (France)
Show Abstract
Synchronized and timing-stabilized pulse generation from a gain-switched laser diode for stimulated Raman scattering microscopy
Paper 9712-20
Time: 4:55 PM - 5:10 PM
Author(s): Kyoya Tokunaga, The Univ. of Tokyo (Japan); Yi-Cheng Fang, Hiroyuki Yokoyama, Tohoku Univ. (Japan); Yasuyuki Ozeki, The Univ. of Tokyo (Japan)
Show Abstract
Session PSun:
Posters-Sunday
Sunday 14 February 2016
5:30 PM - 7:30 PM
Location: Moscone West Levels 2 and 3
  • Session Chairs:
  • Holly Aaron, Univ. of California, Berkeley (United States);
  • Kevin W. Eliceiri, Univ. of Wisconsin-Madison (United States);
  • Alex J. Walsh, Air Force Research Lab. (United States)

Conference attendees are invited to attend the BiOS poster session on Sunday evening. Come view the posters, enjoy light refreshments, ask questions, and network with colleagues in your field. Authors of poster papers will be present to answer questions concerning their papers. Attendees are required to wear their conference registration badges to the poster sessions. Poster authors, view poster presentation guidelines and set-up instructions at http://spie.org/PWPosterGuidelines.
Multiphoton fluorescence lifetime imaging of metabolic status in mesenchymal stem cell during adipogenic differentiation
Paper 9712-28
Time: 5:30 PM - 7:30 PM
Author(s): Aleksandra V. Meleshina, N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation), Nizhny Novgorod State Medical Academy (Russian Federation); Varvara V. Dudenkova, Alena S. Bystrova, Elena I. Cherkasova, N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation); Elena V. Zagaynova, Nizhny Novgorod State Medical Academy (Russian Federation), N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation); Marina V. Shirmanova, Nizhny Novgorod State Medical Academy (Russian Federation)
Show Abstract
Evaluation of collagen by second-harmonic generation microscopy support the heterogeneity of luminal breast cancer
Paper 9712-58
Time: 5:30 PM - 7:30 PM
Author(s): Rodrigo de Andrade Natal, Vitor B. Pelegati, Caroline Bondarik, Luís O. Z. Sarian, Sophie F. M. Derchain, Carlos L. César, José Vassallo, Univ. Estadual de Campinas (Brazil)
Show Abstract
In vivo imaging flow cytometry based on laser scanning two-photon microscopy at kHz cross-sectional frame rate
Paper 9712-59
Time: 5:30 PM - 7:30 PM
Author(s): Lingjie Kong, Purdue Univ. (United States); Jianyong Tang, National Institutes of Health (United States); Meng Cui, Purdue Univ. (United States)
Show Abstract
Comparison of in vivo and ex vivo imaging of the microvasculature with 2-photon fluorescence microscopy
Paper 9712-60
Time: 5:30 PM - 7:30 PM
Author(s): Joe Steinman, Univ. of Toronto (Canada), The Hospital for Sick Children (Canada); Margaret Koletar, Sunnybrook Research Institute (Canada); Bojana Stefanovic, Sunnybrook Research Institute (Canada), Univ. of Toronto (Canada); John G. Sled, Univ. of Toronto (Canada), The Hospital for Sick Children (Canada)
Show Abstract
Four-wave mixing based light sources for real-world biomedical applications of coherent Raman microscopy
Paper 9712-61
Time: 5:30 PM - 7:30 PM
Author(s): Thomas Gottschall, Tobias Meyer, Michael Schmitt, Friedrich-Schiller-Univ. Jena (Germany); Jürgen Popp, Friedrich-Schiller-Univ. Jena (Germany), Leibniz-Institut für Photonische Technologien e.V. (Germany); Jens Limpert, Friedrich-Schiller-Univ. Jena (Germany); Andreas Tünnermann, Friedrich-Schiller-Univ. Jena (Germany), Fraunhofer Institute for Applied Optics and Precision Engineering (Germany)
Show Abstract
Noninvasive visualization of pheomelanin using coherent Raman scattering microscopy
Paper 9712-62
Time: 5:30 PM - 7:30 PM
Author(s): Hequn Wang, Massachusetts General Hospital (United States); Sam Osseiran, Massachusetts Institute of Technology (United States), Harvard-MIT Health Sciences and Technology (HST) Program (United States); Elisabeth Roider, David E. Fisher, Massachusetts General Hospital (United States); Conor L. Evans, Massachusetts General Hospital (United States), Harvard Medical School (United States)
Show Abstract
The nature of multiphoton fluorescence from red blood cells
Paper 9712-63
Time: 5:30 PM - 7:30 PM
Author(s): Ilyas Saytashev, Michigan State Univ. (United States); Michael Murphy, Wellman Ctr. for Photomedicine (United States); Sam Osseiran, Massachusetts Institute of Technology (United States); Dana M. Spence, Michigan State Univ. (United States); Conor L. Evans, Massachusetts General Hospital (United States), Harvard Medical School (United States); Marcos Dantus, Michigan State Univ. (United States)
Show Abstract
Evaluation of oxidative stress in human skin exposed to common sun filters by two-photon excitation fluorescence (2PEF) and fluorescence lifetime imaging microscopy (FLIM)
Paper 9712-64
Time: 5:30 PM - 7:30 PM
Author(s): Sam Osseiran, Yusuke Suita, Elisabeth Roider, Hequn Wang, David E. Fisher, Conor L. Evans, Massachusetts General Hospital (United States)
Show Abstract
Two-photon fluorescence lifetime imaging on a fiber-optic endoscopic platform
Paper 9712-65
Time: 5:30 PM - 7:30 PM
Author(s): Wenxuan Liang, Guanghan Meng, Johns Hopkins Univ. (United States); Israel Gannot, Tel Aviv Univ. (Israel); Ming-Jun Li, Corning Incorporated (United States); Xingde Li, Johns Hopkins Univ. (United States)
Show Abstract
In vivo two-photon imaging measuring the blood-brain barrier permeability during early postnatal brain development in rodent
Paper 9712-66
Time: 5:30 PM - 7:30 PM
Author(s): Lingyan Shi, Adrian Rodriguez-Contreras, The City College of New York (United States)
Show Abstract
Coherent Raman scattering microscopy for probing longitudinal molecular orientations using spirally polarized light
Paper 9712-67
Time: 5:30 PM - 7:30 PM
Author(s): Jian Lin, Zhiwei Huang, National Univ. of Singapore (Singapore)
Show Abstract
Mapping of intracellular concentrations of macromolecules by two-photon excited fluorescence lifetime imaging
Paper 9712-69
Time: 5:30 PM - 7:30 PM
Author(s): Lixin Liu, Xidian Univ. (China); Artem Pliss, Univ. at Buffalo (United States); Xiao Peng, Shenzhen Univ. (China); Andrey N. Kuzmin, Univ. at Buffalo (United States); Junle Qu, Shenzhen Univ. (China); Paras N. Prasad, Univ. at Buffalo (United States)
Show Abstract
Observation of tendon repair in animal model using second-harmonic-generation microscopy
Paper 9712-70
Time: 5:30 PM - 7:30 PM
Author(s): Eiji Hase, Takeo Minamikawa, Katsuya Sato, The Univ. of Tokushima (Japan); Mitsuhiko Takahashi, Takamatsu Red Cross Hospital (Japan); Takeshi Yasui, The Univ. of Tokushima (Japan)
Show Abstract
In situ quantitative evaluation of osteoblastic collagen synthesis under cyclic strain by using second-harmonic-generation microscope
Paper 9712-71
Time: 5:30 PM - 7:30 PM
Author(s): Oki Matsubara, Eiji Hase, Takeo Minamikawa, Takeshi Yasui, Katsuya Sato, The Univ. of Tokushima (Japan)
Show Abstract
Imaging of three-photon induced fluorescence from graphene oxides on the biomimetic phantom
Paper 9712-72
Time: 5:30 PM - 7:30 PM
Author(s): Seung Won Jun, Sang Min Park, Jin Soo Choi, Seok Hee Kang, Suck Won Hong, Chang-Seok Kim, Pusan National Univ. (Korea, Republic of)
Show Abstract
Multi-modal optical microscopy with multiphoton, second-harmonic generation and optical coherence microscopy using supercontinuum generation
Paper 9712-73
Time: 5:30 PM - 7:30 PM
Author(s): Jaehun Kim, Daekeun Kim, Dankook Univ. (Korea, Republic of)
Show Abstract
Observation of two-photon fluorescence spectrum in cellular and tissue structures
Paper 9712-74
Time: 5:30 PM - 7:30 PM
Author(s): Sung-Ho Lee, Bo Ram Kim, Yumee Jeong, Hong-Gyu Ahn, Seung-Han Park, Yonsei Univ. (Korea, Republic of)
Show Abstract
Real time imaging of live cell membrane using laser trapping, reflectance confocal microscopy, and multiphoton fluorescence microscopy
Paper 9712-75
Time: 5:30 PM - 7:30 PM
Author(s): Yunxian Tian, Haishan Zeng, Shangyuan Feng, Yimei Huang, Jianhua Zhao, Eddie Shen, Wenbo Wang, BC Cancer Research Ctr. (Canada); Caigan Du, The Univ. of British Columbia (Canada)
Show Abstract
Stimulated Raman imaging of newly synthesized proteome response under cellular stress
Paper 9712-76
Time: 5:30 PM - 7:30 PM
Author(s): Lixue Shi, Lu Wei, Yihui Shen, Wei Min, Columbia Univ. (United States)
Show Abstract
Nonlinear optical Stokes ellipsometric (NOSE) microscopy for imaging the nonlinear susceptibility tensors of collagen
Paper 9712-77
Time: 5:30 PM - 7:30 PM
Author(s): Ximeng You, Emma L. DeWalt, Purdue Univ. (United States); Shane Z. Sullivan, Purdue University (United States); Paul D. Schmitt, Garth J. Simpson, Purdue Univ. (United States)
Show Abstract
High speed wide field stimulated Raman scattering microscopy with an extreme high full well capacity camera and selective attenuation of background field
Paper 9712-78
Time: 5:30 PM - 7:30 PM
Author(s): Yang-Hyo Kim, Massachusetts Institute of Technology (United States); Jeon Woong Kang, Ramachandra R. Dasari, Laser Biomedical Research Ctr. (United States), Massachusetts Institute of Technology (United States); Shyamsunder Erramilli, Boston Univ. (United States); Peter T. C. So, Massachusetts Institute of Technology (United States)
Show Abstract
Novel pattern matching based fluorophore identification for advanced FLIM analysis
Paper 9712-79
Time: 5:30 PM - 7:30 PM
Author(s): Uwe Ortmann, Benedikt Kraemer, PicoQuant GmbH (Germany); Thomas Niehoerster, Anna Loeschberger, Julius-Maximilians-Univ. Würzburg (Germany); Volker Buschmann, Marcelle Koenig, Paja Reisch, Matthias Patting, Felix Koberling, PicoQuant GmbH (Germany); Ingo Gregor, Georg-August-Univ. Göttingen (Germany); Sandra Orthaus-Müller, Olaf Schulz, Rainer Erdmann, PicoQuant GmbH (Germany)
Show Abstract
Clustering and tracking of single membrane proteins in living bacteria by multi-dimensional microscopy
Paper 9712-80
Time: 5:30 PM - 7:30 PM
Author(s): Daniela Hellwig, Friedrich-Schiller-Univ. Jena (Germany); Gabriele Deckers-Hebestreit, Univ. Osnabrück (Germany); Michael Börsch, Friedrich-Schiller-Univ. Jena (Germany)
Show Abstract
Line-scanning two-photon microscope for in vivo imaging of brain activity
Paper 9712-81
Time: 5:30 PM - 7:30 PM
Author(s): Marie-Pierre Adam, European Lab. for Non-linear Spectroscopy (Italy); Domenico Alfieri, Light4Tech Firenze S.r.l. (Italy); Leonardo Sacconi, Francesco S. Pavone, European Lab. for Non-linear Spectroscopy (Italy)
Show Abstract
Time-gated FLIM microscope for corneal metabolic imaging
Paper 9712-82
Time: 5:30 PM - 7:30 PM
Author(s): Susana Silva, Ana Batista, José Paulo Domingues, Univ. de Coimbra (Portugal); Maria João Quadrado, Univ. de Coimbra (Portugal), Coimbra Hospital and Univ. Ctr. (Portugal); António Miguel Morgado, Univ. de Coimbra (Portugal)
Show Abstract
Higher harmonic generation microscopy of human brain tumors and temporal lobe epilepsy
Paper 9712-83
Time: 5:30 PM - 7:30 PM
Author(s): Nikolay Kuzmin, Vrije Univ. Amsterdam (Netherlands); P. Wesseling, Vrije Univ. Amsterdam (Netherlands), Radboud Univ. Medical Ctr. (Netherlands); P. C. de Witt Hamer, D. P. Noske, Vrije Univ. Medical Ctr. (Netherlands), VUmc Cancer Ctr. Amsterdam (Netherlands); A. J. Rozemuller, Vrije Univ. Medical Ctr. (Netherlands); E. Aronica, Vrije Univ. Medical Ctr. (Netherlands), Stichting Epilepsie Instellingen Nederland - Heemstede (Netherlands); S. Idema, Vrije Univ. Medical Ctr. (Netherlands), VUcm Cancer Ctr. Amsterdam (Netherlands); Giovanni D. Galgano, H. D. Mansvelder, Vrije Univ. Amsterdam (Netherlands); J. C. Baayen, Vrije Univ. Medical Ctr. (Netherlands), Vrije Univ. Amsterdam (Netherlands), VUcm Cancer Ctr. Amsterdam (Netherlands); Marloes L. Groot, Vrije Univ. Amsterdam (Netherlands)
Show Abstract
FLIM data analysis of NADH and tryptophan autofluorescence in prostate cancer cells
Paper 9712-84
Time: 5:30 PM - 7:30 PM
Author(s): Meghan J. O'Melia, Horst K. Wallrabe, Zdenek Svindrych, Shagufta Rehman, Ammasi Periasamy, Univ. of Virginia (United States)
Show Abstract
In-vivo dynamical analysis zonal difference of hepatobiliary metabolism in chronic hepatic diseases by multiphoton microscopy
Paper 9712-85
Time: 5:30 PM - 7:30 PM
Author(s): Chen-Yuan Dong, Chih-Ju Lin, National Taiwan Univ. (Taiwan); Hsuan-Shu Lee, National Taiwan Univ. Hospital (Taiwan)
Show Abstract
Monday 15 February Show All Abstracts
Session 5:
FLIM/FRET/FCS I
Monday 15 February 2016
8:30 AM - 9:35 AM
Location: Room 2014 (West Level 2)
Imaging of calcium transients in cultured neurons by TCSPC FLIM (Invited Paper)
Paper 9712-21
Time: 8:30 AM - 8:55 AM
Author(s): Wolfgang Becker, Becker & Hickl GmbH (Germany); Samuel Frere, Tel Aviv Univ. (Israel)
Show Abstract
Binding of the immunomodulatory drug Bz-423 to mitochondrial FoF1-ATP synthase in living cells by FLIM-FRET and FRET acceptor photobleaching (Invited Paper)
Paper 9712-22
Time: 8:55 AM - 9:20 AM
Author(s): Ilka Starke, Friedrich-Schiller-Univ. Jena (Germany); Gary D. Glick, Univ. of Michigan (United States); Michael Börsch, Friedrich-Schiller-Univ. Jena (Germany)
Show Abstract
Investigation of prostate cancer cells using NADH and Tryptophan as biomarker: multiphoton FLIM-FRET microscopy
Paper 9712-23
Time: 9:20 AM - 9:35 AM
Author(s): Shagufta Rehman, Meghan J. O'Melia, Univ. of Virginia (United States); Horst Wallrabe, Univ of Virginia (United States); Zdenek Svindrych, Univ. of Virginia (United States); Dhyan Chandra, Roswell Park Cancer Institute (United States); Ammasi Periasamy, Univ. of Virginia (United States)
Show Abstract
Session 6:
JenLab Young Investigator Award Papers Presentation
Monday 15 February 2016
9:35 AM - 10:05 AM
Location: Room 2014 (West Level 2)
Coffee Break 10:05 AM - 10:30 AM
Session 7:
FLIM/FRET/FCS II
Monday 15 February 2016
10:30 AM - 12:00 PM
Location: Room 2014 (West Level 2)
Temporal and spatial binning of TCSPC data to improve signal-to-noise ratio and imaging speed (Invited Paper)
Paper 9712-24
Time: 10:30 AM - 10:55 AM
Author(s): Alex J. Walsh, National Research Council (United States), Air Force Research Lab. (United States); Hope T. Beier, Air Force Research Lab. (United States)
Show Abstract
Tunable PIE and synchronized gating detections by FastFLIM for quantitative microscopy measurements of fast dynamics of single molecules (Invited Paper)
Paper 9712-25
Time: 10:55 AM - 11:20 AM
Author(s): Yuansheng Sun, Ulas Coskun, ISS, Inc. (United States); Allan Chris Ferreon, Department of pharmacology, Baylor College of Medicine (United States); Beniamino B. Barbieri, Shih-Chu Liao, ISS, Inc. (United States)
Show Abstract
ns-time resolution for multispecies STED-FLIM and artifact free STED-FCS (Invited Paper)
Paper 9712-26
Time: 11:20 AM - 11:45 AM
Author(s): Marcelle Koenig, Paja Reisch, Rhys Dowler, Benedikt Kraemer, Sebastian Tannert, Matthias Patting, PicoQuant GmbH (Germany); Christian Eggeling, MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford (United Kingdom); Felix Koberling, Rainer Erdmann, PicoQuant GmbH (Germany)
Show Abstract
Live-tracking drug delivery in skin: multiphoton FLIM FRET
(Canceled)
Paper 9712-27
Time: 11:45 AM - 12:00 PM
Author(s):
Show Abstract
Lunch Break 12:00 PM - 1:45 PM
Session 8:
Technology Development I
Monday 15 February 2016
1:45 PM - 2:30 PM
Location: Room 2014 (West Level 2)
  • Session Chair:
  • Peter T. C. So, Massachusetts Institute of Technology (United States)
Metabolic microscopy of head and neck cancer organoids
Paper 9712-29
Time: 1:45 PM - 2:00 PM
Author(s): Amy T. Shah, Melissa C. Skala, Vanderbilt Univ. (United States)
Show Abstract
STED FLIM: fluorescence lifetime imaging with 30nm resolution
(Canceled)
Paper 9712-30
Time: 2:00 PM - 2:15 PM
Author(s):
Show Abstract
Using FLIM to characterize liver damage and solute disposition in vivo
Paper 9712-86
Time: 2:00 PM - 2:15 PM
Author(s): Camilla Anngelie Thorling, The Univ. of Queensland School of Medicine (Australia); Hauke Studier, Univ. of South Australia (Australia); Xin Liu, The Univ. of Queensland School of Medicine (Australia); Frank J. Burczynski, Univ. of Manitoba (Canada); Darrell Crawford, The Univ. of Queensland School of Medicine (Australia); Michael S. Roberts, The Univ. of Queensland School of Medicine (Australia), Univ. of South Australia (Australia)
Show Abstract
A phasor approach analysis of multiphoton FLIM measurements of three-dimensional cell culture models
Paper 9712-31
Time: 2:15 PM - 2:30 PM
Author(s): Pirmin Lakner, Universitätsklinikum Tübingen (Germany); Yvonne Möller, Monilola Olayioye, Univ. Stuttgart (Germany); Sara Y. Brucker, Eberhard Karls Univ Tübingen, Univ Hospital, Dept of Women's Health (Germany); Katja Schenke-Layland, Universitätsklinikum Tübingen (Germany), Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik (Germany); Michael G. Monaghan, Universitätsklinikum Tübingen (Germany)
Show Abstract
Award Presentation
Monday 15 February 2016
2:30 PM - 3:00 PM
Location: Room 2014 (West Level 2)

Student Poster Session Competition

Prize donated by:
Becker and Hickl, Carl Zeiss Microscopy, LLC, Chroma Tech, Coherent Inc., ISS Inc., Leica Microsystems, Newport Corporation, Semrock

Graduate students and postdoctoral fellows are welcome to participate in the poster session competition of the conference on Multiphoton Microscopy in the Biomedical Sciences. There is a cash award for the winner(s). The winner(s) will be informed in person or by email and must receive the award in person in the conference hall. Participants should follow the rules and regulations of SPIE for submission of their abstract and manuscript. Participants should also register their names for the competition with the Conference Chairs during the first day of the conference.


JenLab Young Investigator Award

Prize donated by:
JenLab GmbH

We are pleased to announce that a prize in the amount of $2000.00 will be awarded to a graduate student, postdoc, scientist, or junior faculty under the age of 32. To be eligible to receive the award, participants must 1) be both the primary author and presenter of an accepted abstract, 2) submit the proceedings paper by the due date, prior to the meeting, for review by the selection committee, 3) submit their paper for consideration.
Coffee Break 3:00 PM - 3:30 PM
Session 9:
Technology Development II
Monday 15 February 2016
3:30 PM - 5:05 PM
Location: Room 2014 (West Level 2)
  • Session Chair:
  • Peter T. C. So, Massachusetts Institute of Technology (United States)
High-throughput 3D super-resolution imaging by volumetric parallel STED microscopy (Invited Paper)
Paper 9712-32
Time: 3:30 PM - 3:55 PM
Author(s): Yi Xue, Christopher J. Rowlands, Peter T. C. So, Massachusetts Institute of Technology (United States)
Show Abstract
SearchLight: a freely available web-based quantitative spectral analysis tool (Invited Paper)
Paper 9712-33
Time: 3:55 PM - 4:20 PM
Author(s): Prashant Prabhat, Michael Peet, Turan Erdogan, Semrock, Inc. (United States)
Show Abstract
Recent developments in widely tunable and high peak power ultrafast laser sources and their adoption in biological imaging
Paper 9712-34
Time: 4:20 PM - 4:35 PM
Author(s): Julien Klein, Spectra-Physics (United States)
Show Abstract
Advanced femtosecond lasers enable new developments in non-linear imaging and functional studies in neuroscience, biology and medical applications
Paper 9712-35
Time: 4:35 PM - 4:50 PM
Author(s): Marco Arrigoni, Coherent, Inc. (United States); Darryl McCoy, Coherent Scotland Ltd. (United Kingdom)
Show Abstract
Airyscan detection combined with two-photon excitation improves imaging signal-to-noise and resolution in thick scattering samples
(Canceled)
Paper 9712-36
Time: 4:50 PM - 5:05 PM
Author(s):
Show Abstract
Six wave mixing coherent anti-stokes Raman scattering (CARS) microscopy
Paper 9712-87
Time: 4:50 PM - 5:05 PM
Author(s): Vitor B. Pelegati, Bernardo B. C. Kyotoku, Lazaro A. Padilha, Instituto de Física "Gleb Wataghin" (Brazil)
Show Abstract
Tuesday 16 February Show All Abstracts
Session 10:
Second/Third Harmonic Generation I
Tuesday 16 February 2016
8:30 AM - 9:55 AM
Location: Room 2014 (West Level 2)
Analysis of stromal alterations in ovarian cancers via wavelength dependent second harmonic generation microscopy and optical scattering (Invited Paper)
Paper 9712-37
Time: 8:30 AM - 8:55 AM
Author(s): Paul J. Campagnola, Karissa B. Tilbury, Kirby R. Campbell, Kevin W. Eliceiri, Mansih Patankar, Univ. of Wisconsin-Madison (United States)
Show Abstract
Volumetric imaging of oral epithelial neoplasia by MPM-SHGM: epithelial connective tissue interface
Paper 9712-38
Time: 8:55 AM - 9:10 AM
Author(s): Rahul Pal, Jinping Yang, Suimin Qiu, Vicente Resto, Susan McCammon, Gracie Vargas, The Univ. of Texas Medical Branch (United States)
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Characterization of human arterial tissue affected by atherosclerosis using multimodal nonlinear optical microscopy
Paper 9712-39
Time: 9:10 AM - 9:25 AM
Author(s): Enrico Baria, European Lab. for Non-linear Spectroscopy (Italy); Riccardo Cicchi, European Lab. for Non-linear Spectroscopy (Italy), Istituto Nazionale di Ottica (Italy); Matteo Rotellini, Univ. degli Studi di Firenze (Italy); Gabriella Nesi, Division of Pathology, Department of Surgery and Translational Medicine, University of Florence (Italy); Daniela Massi, Univ. degli Studi di Firenze (Italy); Francesco S. Pavone, European Lab. for Non-linear Spectroscopy (Italy), Istituto Nazionale di Ottica (Italy), Univ. degli Studi di Firenze (Italy)
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Forward versus backward polarization-resolved SHG imaging of collagen structure in tissues
Paper 9712-40
Time: 9:25 AM - 9:40 AM
Author(s): Claire Teulon, Ecole Polytechnique (France), Consiglio Nazionale delle Ricerche - Inserm U1182 (France); Ivan Gusachenko, Ecole Polytechnique (France), Consiglio Nazionale delle Ricerche - Inserm U1182 (France), Okinawa Institute of Science and Technology Graduate Univ. (Japan); Gaël Latour, Ecole Polytechnique (France), Consiglio Nazionale delle Ricerche - Inserm U1182 (France), Univ. Paris-Sud 11 (France); Marie-Claire Schanne-Klein, Lab. d'Optique et Biosciences (France), Consiglio Nazionale delle Ricerche - Inserm U1182 (France)
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Impact of relative collagen fibril polarity in determining the signal intensity in second harmonic generation microscopy
Paper 9712-41
Time: 9:40 AM - 9:55 AM
Author(s): Charles-André Couture, Stéphane Bancelin, Institut National de la Recherche Scientifique (Canada); Jarno N. Van der Kolk, Konstantin Popov, Univ. of Ottawa (Canada); Maxime Rivard, Katherine Légaré, Institut National de la Recherche Scientifique (Canada); Gabrielle Martel, Univ. de Montréal (Canada); Hélène Richard, Univ. of Ottawa (Canada); Cameron Brown, Univ. of Oxford (United Kingdom); Sheila Laverty, Univ. de Montréal (Canada); Lora Ramunno, Univ. of Ottawa (Canada); François Légaré, Institut National de la Recherche Scientifique (Canada)
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Coffee Break 9:55 AM - 10:25 AM
Session 11:
Second/Third Harmonic Generation II
Tuesday 16 February 2016
10:25 AM - 12:00 PM
Location: Room 2014 (West Level 2)
Two-photon imaging during brain surgery: first clinical study using a certified multiphoton tomograph (Invited Paper)
Paper 9712-42
Time: 10:25 AM - 10:50 AM
Author(s): Karsten König, Univ. des Saarlandes (Germany), JenLab GmbH (Germany); Martin Weinigel, JenLab GmbH (Germany); Sven R. Kantelhardt, Alf Giese, Universitätsmedizin der Johannes Gutenberg-Univ. Mainz (Germany)
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Biocompatibility of novel ultracompact femtosecond laser oscillators for multiphoton imaging (Invited Paper)
Paper 9712-43
Time: 10:50 AM - 11:15 AM
Author(s): Aisada Uchugonova, Univ. des Saarlandes (Germany), JenLab GmbH (Germany); Hans G. Breunig, JenLab GmbH (Germany); Tuan Li, FEMTOLASERS Produktions GmbH (Austria); Karsten König, Univ. des Saarlandes (Germany), JenLab GmbH (Germany)
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Multimodal imaging platform to study cancer progression in zebrafish
Paper 9712-44
Time: 11:15 AM - 11:30 AM
Author(s): Angelika Unterhuber, Marco Andreana, Aart J. Verhoef, Medizinische Univ. Wien (Austria); Martin Distel, St. Anna Kinderkrebsforschung e.V. (Austria); Alma del Carmen Fernandez Gonzalez, Technische Univ. Wien (Austria); René M. Werkmeister, Wolfgang Drexler, Medizinische Univ. Wien (Austria)
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Multiphoton imaging with a nanosecond supercontinuum source
Paper 9712-45
Time: 11:30 AM - 11:45 AM
Author(s): Claire Lefort, XLIM Institut de Recherche (France), Univ. de Limoges (France), Consiglio Nazionale delle Ricerche (France); Rodney P. O'Connor, XLIM Institut de Recherche (France); Véronique Blanquet, Fabienne Baraige, Unité de Génétique Moléculaire Animale (France); Vincent Tombelaine, LEUKOS (France); Philippe Leveque, Vincent Couderc, Philippe Leproux, XLIM Institut de Recherche (France)
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Coherence gated wavefront sensorless adaptive optics for two photon imaging
Paper 9712-46
Time: 11:45 AM - 12:00 PM
Author(s): Yifan Jian, Michelle Cua, Simon Fraser Univ. (Canada); Stefano Bonora, IFN-CNR LUXOR Lab. (Italy); Edward N. Pugh, Robert J. Zawadzki, Univ. of California, Davis (United States); Marinko V. Sarunic, Simon Fraser Univ. (Canada)
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Lunch Break 12:00 PM - 1:30 PM
Session 12:
Technology Development III
Tuesday 16 February 2016
1:30 PM - 3:05 PM
Location: Room 2014 (West Level 2)
  • Session Chair:
  • Francesco S. Pavone, European Lab. for Non-linear Spectroscopy (Italy)
Large field of view in vivo multiphoton microscopy of human skin (Invited Paper)
Paper 9712-47
Time: 1:30 PM - 1:55 PM
Author(s): Mihaela Balu, Beckman Laser Institute and Medical Clinic (United States); Hideharu Mikami, The Univ. of Tokyo (Japan), Univ. of California, Irvine (United States); Jue Hou, Beckman Laser Institute and Medical Clinic (United States); Eric O. Potma, Univ. of California, Irvine (United States); Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)
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Design of a portable, wide field of view, GPU-accelerated multiphoton imaging system for real-time imaging of breast surgical specimens (Invited Paper)
Paper 9712-48
Time: 1:55 PM - 2:20 PM
Author(s): Michael G. Giacomelli, Tadayuki Yoshitake, Osman O. Ahsen, Massachusetts Institute of Technology (United States); Lennart A. Husvogt, Friedrich-Alexander-Univ. Erlangen-Nürnberg (Germany); Yury Sheykin, Hilde Vardeh, Beth Israel Deaconess Medical Ctr. (United States); Jeffrey Brooker, Thorlabs, Inc. (United States); James L. Connolly, Beth Israel Deaconess Medical Ctr. (United States); Joachim Hornegger, Friedrich-Alexander-Univ. Erlangen-Nürnberg (Germany); Alex E. Cable, Thorlabs, Inc. (United States); James G. Fujimoto, Massachusetts Institute of Technology (United States)
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Compact fixed wavelength femtosecond oscillators as an add-on for tunable titanium sapphire lases extend the range of applications towards multimodal imaging and optogenetics
Paper 9712-49
Time: 2:20 PM - 2:35 PM
Author(s): Tommi Hakulinen, Spectra-Physics (Austria); Julien Klein, Spectra-Physics (United States)
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Advanced multiphoton methods for in vitro and in vivo functional imaging of mouse retinal neurons
Paper 9712-50
Time: 2:35 PM - 2:50 PM
Author(s): Noam Cohen, Adi Schejter, Nairouz Farah, Shy Shoham, Technion-Israel Institute of Technology (Israel)
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Widefield three-photon excitation: excitation of quantum dots and optogenetic opsins using 1300nm excitation
Paper 9712-51
Time: 2:50 PM - 3:05 PM
Author(s): Christopher J. Rowlands, Demian Park, Kiryl Piatkevich, Oliver T. Bruns, Moungi G. Bawendi, Edward S. Boyden, Peter T. C. So, Massachusetts Institute of Technology (United States)
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Coffee Break 3:05 PM - 3:30 PM
Session 13:
Technology Development IV
Tuesday 16 February 2016
3:30 PM - 5:10 PM
Location: Room 2014 (West Level 2)
Label-free NIR reflectance imaging as a complimentary tool for two-photon fluorescence microscopy (Invited Paper)
Paper 9712-52
Time: 3:30 PM - 3:55 PM
Author(s): Anna Letizia Allegra Mascaro, European Lab. for Non-linear Spectroscopy (Italy), Consiglio Nazionale delle Ricerche (Italy), Istituto Nazionale di Ottica (Italy); Irene Costantini, Emilia Margoni, European Lab. for Non-linear Spectroscopy (Italy); Giulio Iannello, Alessandro Bria, Univ. Campus Bio-Medico (Italy); Leonardo Sacconi, European Lab. for Non-linear Spectroscopy (Italy), Consiglio Nazionale delle Ricerche (Italy), Istituto Nazionale di Ottica (Italy); Francesco S. Pavone, European Lab. for Non-linear Spectroscopy (Italy), Univ. degli Studi di Firenze (Italy)
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Brillouin imaging/sensing via time-resolved optical (BISTRO) measurements
Paper 9712-53
Time: 3:55 PM - 4:10 PM
Author(s): Zhaokai Meng, Charles Ballman, Georgi I. Petrov, Vladislav V. Yakovlev, Texas A&M Univ. (United States)
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Multi-photon microscope driven by novel green laser pump
Paper 9712-54
Time: 4:10 PM - 4:25 PM
Author(s): Dominik Marti, DTU Risø Campus (Denmark); Martin Djurhuus, Technical Univ. of Denmark (Denmark); Ole B. Jensen, Peter E. Andersen, DTU Risø Campus (Denmark)
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Ratiometric detection of O2 sensing probe in tumor model via two photon microscopy
Paper 9712-55
Time: 4:25 PM - 4:40 PM
Author(s): Calvin J. Yoon, Bumju Kim, Junhwa Lee, Seoyeon Bok, Joonhyuck Park, Seunghun Lee, Viet Hoan Le, Sungjee Kim, G-One Ahn, Ki Hean Kim, Pohang Univ. of Science and Technology (Korea, Republic of)
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A new method using multiphoton imaging and morphometric analysis for differentiating chromophobe renal cell carcinoma and oncocytoma kidney cancers
Paper 9712-56
Time: 4:40 PM - 4:55 PM
Author(s): Binlin Wu, Southern Connecticut State Univ. (United States); Sushmita Mukherjee, Manu Jain, Weill Cornell Medical College (United States)
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Quantitative structural markers of colorectal dysplasia in a cross sectional study of ex vivo murine tissue using label-free multiphoton microscopy
Paper 9712-57
Time: 4:55 PM - 5:10 PM
Author(s): Sandra P. Prieto, Gage J. Greening, Univ. of Arkansas (United States); Keith K. Lai, Univ. of Arkansas for Medical Sciences (United States); Timothy J. Muldoon, Univ. of Arkansas (United States)
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