The Moscone Center
San Francisco, California, United States
7 - 12 February 2015
Conference 9329
Multiphoton Microscopy in the Biomedical Sciences XV
Sunday - Tuesday 8 - 10 February 2015
Conference
Committee
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Conference Chairs
  • Ammasi Periasamy, Univ. of Virginia (United States)
  • Peter T. So, Massachusetts Institute of Technology (United States)
  • Karsten König, Univ. des Saarlandes (Germany)

Program Committee
Program Committee continued...
Sunday 8 February Show All Abstracts
Opening Remarks
Sunday 8 February 2015
8:15 AM - 8:30 AM

Ammasi Periasamy, Univ. of Virginia (USA)
Session 1:
Keynote Session
Sunday 8 February 2015
8:30 AM - 10:00 AM
Some new directions for multiphoton processes and their biomedical applications (Keynote Presentation)
Paper 9329-1
Author(s): Paras N. Prasad, Univ. at Buffalo (United States)
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Multiphoton microscopy in brain imaging (Keynote Presentation)
Paper 9329-2
Author(s): Francesco S. Pavone, European Lab. for Non-linear Spectroscopy (Italy)
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Fluorescence lifetime imaging: techniques and applications (Keynote Presentation)
Paper 9329-3
Author(s): Wolfgang Becker, Becker & Hickl GmbH (Germany)
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Session 2:
FLIM/FRET/FCS I
Sunday 8 February 2015
10:30 AM - 12:25 PM
Fluorescence polarization and fluctuation analysis (FPFA): a method for simultaneously measuring fluorescence lifetime, time-resolved anisotropy, fluorophore concentration, molecular brightness, and lateral diffusion times (Invited Paper)
Paper 9329-4
Author(s): Tuan A. Nguyen, Jithesh V. Veetil, Pabak Sarkar, Steven S. Vogel, National Institutes of Health (United States)
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Recent advances in pattern matching based multi-species FLIM analysis (Invited Paper)
Paper 9329-5
Author(s): Benedikt Krämer, PicoQuant GmbH (Germany); Thomas Niehörster, Anna Löschberger, Julius-Maximilians-Univ. Würzburg (Germany); Marcelle König, PicoQuant GmbH (Germany); Paja Reisch, Picoquant GmbH (Germany); Matthias Patting, Felix Koberling, PicoQuant GmbH (Germany); Ingo Gregor, Georg-August-Univ. Göttingen (Germany); Markus Sauer, Julius-Maximilians-Univ. Würzburg (Germany); Rainer Erdmann, PicoQuant GmbH (Germany)
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Application of quantitative fluorescence imaging techniques for investigating intracellular interactions and dynamics of HIV-1 proteins
Paper 9329-6
Author(s): Yves Mely, Halina Anton, Nedal Taha, Marianna Sholokh, Salah El Meshri, Univ. de Strasbourg (France); Pascal Didier, Univ. of Strasbourg (France); Emmanuel Boutant, Ludovic Richert, Eleonore Real, Univ. de Strasbourg (France); Hugues de Rocquigny, Univ. of Strasbourg (France)
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New dimensions in TCSPC FLIM: recording transient lifetime effects, spatial mosaics, simultaneous FLIM/PLIM
Paper 9329-7
Author(s): Hauke Studier, Wolfgang Becker, Becker & Hickl GmbH (Germany)
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Fiber-optic two-photon endomicroscopic fluorescence lifetime and fluorescence correlation spectroscopy imaging
Paper 9329-8
Author(s): Wenxuan Liang, Johns Hopkins Univ. (United States); Guanghan Meng, Shanghai Jiao Tong Univ. (China); Gunnsteinn Hall, Johns Hopkins Univ. (United States); Ming-Jun Li, Corning Incorporated (United States); Xingde Li, Johns Hopkins Univ. (United States)
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Observing conformational dynamics of single membrane transporters in a fast anti-Brownian electrokinetic trap
Paper 9329-9
Author(s): Bertram Su, Thomas Heitkamp, Universitätsklinikum Jena (Germany); Monika G. Düser, Nawid Zarrabi, Univ. Stuttgart (Germany); Michael Börsch, Friedrich-Schiller-Univ. Jena (Germany), Abbe Ctr. of Photonics Jena (Germany)
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Imaging of oxygenation in 3D tissue models with multi-modal phosphorescent probes
Paper 9329-97
Author(s): Dmitri B. Papkovsky, Ruslan Dmitriev, Univ. College Cork (Ireland)
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Lunch Break 12:25 PM - 1:30 PM
Session 3:
FLIM/FRET/FCS II
Sunday 8 February 2015
1:30 PM - 3:05 PM
Cell metabolism, FLIM and PLIM and applications (Invited Paper)
Paper 9329-10
Author(s): Angelika C. Rueck, Sviatlana Kalinina, Univ. Ulm (Germany)
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Flavin fluorescence lifetime imaging of living cells grown on structured surfaces
Paper 9329-11
Author(s): Alzbeta Marcek Chorvatova, Julia Horilova, Tibor Teplicky, Beata Cunderlikova, Jaroslav Bruncko, Anton Mateasik, Dusan Chorvat, International Laser Ctr. (Slovakia)
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Characterization of porcine eye based on autofluorescence lifetime imaging
Paper 9329-12
Author(s): Ana Batista, Univ. de Coimbra (Portugal), Saarland Univ. (Germany); Hans Georg Breunig, Univ. des Saarlandes (Germany), JenLab GmbH (Germany); Aisada Uchugonova, Univ. des Saarlandes (Germany); António Miguel Morgado, Univ. de Coimbra (Portugal); Karsten König, Univ. des Saarlandes (Germany), JenLab GmbH (Germany)
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Time-resolved anisotropy imaging with a single detector
Paper 9329-13
Author(s): Vladislav I. Shcheslavskiy, Wolfgang Becker, Becker & Hickl GmbH (Germany)
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Time-resolved spectral imaging: better photon economy, higher accuracy
Paper 9329-14
Author(s): Farzad Fereidouni, Univ. of California, Davis (United States); Keimpe Reitsma, Micro-Key BV (Netherlands); Gerhard A. Blab, Hans C. Gerritsen, Utrecht Univ. (Netherlands)
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TCSPC FLIM for clinical applications: technical solutions and first results
Paper 9329-15
Author(s): Alexander Jelzow, Wolfgang Becker, Becker & Hickl GmbH (Germany); Yoshihiko Katayama, Heidelberg Engineering GmbH (Germany); Karsten König, Univ. des Saarlandes (Germany); Dietrich Schweitzer, Friedrich-Schiller-Univ. Jena (Germany)
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Session 4:
Technology Development and Application I
Sunday 8 February 2015
3:30 PM - 5:25 PM
  • Session Chair:
  • Karsten König, Univ. des Saarlandes (Germany)
Smart 2PE (Invited Paper)
Paper 9329-16
Author(s): Alberto Diaspro, Istituto Italiano di Tecnologia (Italy), Univ. degli Studi di Genova (Italy), Nikon Imaging Ctr. at Fondazione Istituto Italiano di Tecnologia (Italy); Paolo Bianchini, Francesca Cella Zanacchi, Martì Duocastella, Luca L. Lanzanò, Giuseppe Vicidomini, Istituto Italiano di Tecnologia (Italy)
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Deep two-photon microscopic imaging through brain tissue using the second singlet state from fluorescent agent (Invited Paper)
Paper 9329-17
Author(s): Lingyan Shi, The City College of New York (United States)
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Next generation laser sources for non-linear imaging
Paper 9329-18
Author(s): Darryl McCoy, Coherent Scotland Ltd. (United Kingdom); Marco Arrigoni, Nigel Gallaher, Coherent, Inc. (United States)
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Latest advances in ultra-fast laser sources for multi photon microscopy
Paper 9329-19
Author(s): Phil Smith, Newport Corp. (United States)
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Fluorescence endoscopic imaging using a GRIN lens
Paper 9329-20
Author(s): Wei Yan, Xiao Peng, Qi Wang, Jian Gao, Jie Zhou, Bing Yu, Junle Qu, Shenzhen Univ. (China)
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Two-photon excited fluorescence emission from hemoglobin
Paper 9329-21
Author(s): Qiqi Sun, Yan Zeng, Wei Zhang, Hong Kong Univ. of Science and Technology (Hong Kong, China); Wei Zheng, Shenzhen Institute of Advanced Technology (China); Yi Luo, Univ. of Science and Technology of China (China); Jianan Qu, Hong Kong Univ. of Science and Technology (Hong Kong, China)
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In vivo pump-probe microscopy of melanoma: characterizing shifts in excited state photodynamics with respect to invasiveness and chemotherapy response
Paper 9329-22
Author(s): Jesse W. Wilson, Simone Degan, Christina S. Gainey, Tanya Mitropoulos, Mary Jane Simpson, Sanghamitra Deb, Christopher P. Dall, Yasmine Tameze, Jennifer . Zhang, Warren S. Warren, Duke Univ. (United States)
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Session PSun:
Posters-Sunday
Sunday 8 February 2015
5:30 PM - 7:30 PM

Conference attendees are invited to attend the BiOS poster session on Sunday evening. Come view the posters, enjoy light refreshments, ask questions, and network with colleagues in your field. Authors of poster papers will be present to answer questions concerning their papers. Attendees are required to wear their conference registration badges to the poster sessions. Poster authors, view poster presentation guidelines and set-up instructions at http://spie.org/PWPosterGuidelines.
Super resolution brain imaging by using a two-photon fluorescence microscopy with harmonic modulation
Paper 9329-73
Author(s): Szu-Yu Lee, National Taiwan Univ. (Taiwan); Chi-Kuang Sun, National Taiwan Univ. (Taiwan), Academia Sinica (Taiwan), Molecular Imaging Ctr. (Taiwan)
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Fiber based ultra-short pulse delivery for nonlinear spectroscopy and microscopy using high energy solitons
Paper 9329-74
Author(s): Sarah Saint-Jalm, Esben R. Andresen, Institut Fresnel (France); Abdelkrim Bendahmane, PhLAM (France); Alexandre Kudlinski, Lab. de Physique des Lasers, Atomes et Molécules (France); Hervé Rigneault, Institut Fresnel (France)
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Interferometric second harmonic generation imaging of biological tissues
Paper 9329-75
Author(s): Charles-André Couture, Maxime Rivard, Institut National de la Recherche Scientifique (Canada); Konstatin Popov, Univ. of Ottawa (Canada); Mathieu Laliberté, Anthony Bertrand-Grenier, Institut National de la Recherche Scientifique (Canada); Amir Miri, McGill Univ. (Canada); François Martin, Henri Pépin, Institut National de la Recherche Scientifique (Canada); Luc Mongeau, McGill Univ. (Canada); Christian Pfeffer, Ludwig-Maximilians-Univ. (Germany); Cameron Brown, Univ. of Oxford (United Kingdom); Lora Ramunno, Univ. of Ottawa (Canada); François Légaré, Institut National de la Recherche Scientifique (Canada)
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FRAP (fluorescence recovery after photobleaching) imaging using SLM-controlled doughnut spots
Paper 9329-76
Author(s): Yi Xue, Christopher J. Rowlands, Peter T. C. So, Massachusetts Institute of Technology (United States)
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New insights and system designs for temporally-focused multiphoton optogenetics
Paper 9329-77
Author(s): Tom Mayblum, Adi Schejter, Technion-Israel Institute of Technology (Israel); Hod Dana, Janelia Farm Research Campus, Howard Hughes Medical Institute (United States); Shy Shoham, Technion-Israel Institute of Technology (Israel)
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Effects of anesthesia on the cerebral capillary blood flow in young and old mice
Paper 9329-78
Author(s): Mohammad Moeini, Ecole Polytechnique de Montréal (Canada), Montréal Heart Institute (Canada); Maryam S. Tabatabaei, Ecole Polytechnique de Montréal (Canada), Montreal Heart Institute (Canada); Samuel Bélanger, Pramod Avti, Alexandre Castonguay, Philippe Pouliot, Frédéric Lesage, Ecole Polytechnique de Montréal (Canada), Montréal Heart Institute (Canada)
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Determination of the spectral dependence of reduced scattering and quantitative SHG imaging for detection of fibrillar changes in ovarian cancer
Paper 9329-79
Author(s): Kirby R. Campbell, Paul J. Campagnola, Karissa B. Tilbury, Univ. of Wisconsin-Madison (United States)
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Quantitative metabolic imaging captures cellular heterogeneity in head and neck cancer organoids
Paper 9329-80
Author(s): Amy T. Shah, Alex J. Walsh, Melissa C. Skala, Vanderbilt Univ. (United States)
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A modular two-photon microscope for simultaneous imaging of distant cortical areas in vivo
Paper 9329-81
Author(s): Fabian F. Voigt, Jerry L. Chen, Roland Krueppel, Fritjof Helmchen, Univ. Zürich (Switzerland)
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Fluorescence lifetime imaging in the near-infrared region
Paper 9329-82
Author(s): Wolfgang Becker, Vladislav I. Shcheslavskiy, Becker & Hickl GmbH (Germany)
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False positives in SRS imaging of lipid droplets: identification and reduction
Paper 9329-83
Author(s): Xuesong Li, Wen Jiun Lam, Yan Hao, Qiqi Sun, Sicong He, Yan Zeng, Ho Yi Mak, Jianan Qu, Hong Kong Univ. of Science and Technology (Hong Kong, China)
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Classification and recognition of texture collagen obtaining by multiphoton microscope with neural network analysis tool
Paper 9329-84
Author(s): Shulian Wu, Hui Li, Xiaoman Zhang, Fujian Normal Univ. (China)
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Improvement of the spatial resolution in multiphoton microscopy by saturated excitation of fluorescence
Paper 9329-85
Author(s): Anh Dung Nguyen, François Duport, Univ. Libre de Bruxelles (Belgium); Frédérique Vanholsbeeck, The Univ. of Auckland (New Zealand); Serge Goldman, Philippe Emplit, Simon-Pierre Gorza, Univ. Libre de Bruxelles (Belgium)
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Quantitative 3D molecular cutaneous absorption in human skin using label free nonlinear microscopy
Paper 9329-86
Author(s): Xueqin Chen, Institut Fresnel (France); Sébastien Grégoire, Florian Formanek, Jean-Baptiste Galey, L'Oréal (France); Hervé Rigneault, Institut Fresnel (France)
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Statistical analysis on activation and photo-bleaching of step-wise multi-photon activation fluorescence of melanin
Paper 9329-87
Author(s): Zetong Gu, Zhenhua Lai, Xi Zhang, Northeastern Univ. (United States); Jihao Yin, Beihang Univ. (China); Charles A. DiMarzio, Northeastern Univ. (United States)
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Apertureless confocal scanning microscopy under temporally controlled femtosecond pulse illumination
Paper 9329-88
Author(s): Dhiman Das, Soumendra N. Bandyopadhyaya, Debabrata Goswami, Indian Institute of Technology Kanpur (India)
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Multi-photon laser scanning omnidirectional imaging with tunable frame rate
Paper 9329-89
Author(s): Dapeng Zhang, Jiyi Cheng, Shih-Chi Chen, The Chinese Univ. of Hong Kong (Hong Kong, China)
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High-resolution nonlinear ellipse rotation measurements for 3D microscopy
Paper 9329-90
Author(s): Lino Misoguti, Maria L. Miguez, Emerson C. Barbano, Sergio C. Zilio, Univ. de São Paulo (Brazil)
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In situ determination of collagen fibrils size via absolute measurements of second harmonic generation
Paper 9329-91
Author(s): Stéphane Bancelin, Institut National de la Recherche Scientifique (Canada); Carole Aimé, Lab. de Chimie de la Matière Condensée de Paris (France); Ivan Gusachenko, Lab. d’Optique et Biosciences, Ecole Polytechnique (France); Laura Kowalczuk, Therapeutic Innovations (France); Gaël Latour, Lab. d’Optique et Biosciences, Institut d'Électronique Fondamentale (France); Thibaud Coradin, Lab. de Chimie de la Matière Condensée de Paris (France); Marie-Claire Schanne-Klein, Lab. d'Optique et Biosciences, Ecole Polytechnique (France)
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2D simultaneous spatial and temporal focusing multiphoton microscopy for fast volume imaging with improved sectioning ability
Paper 9329-92
Author(s): Qiyuan Song, Keio Univ. (Japan), RIKEN (Japan); Keisuke Isobe, RIKEN (Japan); Kenichi Hirosawa, Keio Univ. (Japan); Katsumi Midorikawa, RIKEN (Japan); Fumihiko Kannari, Keio Univ. (Japan)
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Optimizing laser and probe molecules for multi-photon microscopy
Paper 9329-93
Author(s): Mary J. Potasek, Gene Parilov, Karl Beeson, Simphotek Inc. (United States)
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Motion-free polarization-resolved second harmonic generation cornea imaging with sub-15 fs pulses
Paper 9329-94
Author(s): Hans Georg Breunig, Ana Batista, Aisada Uchugonova, Univ. des Saarlandes (Germany); Karsten König, Univ. des Saarlandes (Germany), JenLab GmbH (Germany)
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In vivo visualization of collagen fiber produced by cultured osteoblasts using sensitive second-harmonic-generation microscopy equipped with a 10-fs mode-locked Ti:sapphire laser
Paper 9329-95
Author(s): Eiji Hase, Katsuya Sato, Takeshi Yasui, Univ. of Tokushima (Japan)
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Modeling acousto-optic two-photon ophthalmoscopy in pseudophakic patients
Paper 9329-96
Author(s): Akos Kusnyerik, Semmelweis Univ. (Hungary); Balazs J. Rozsa, Institute of Experimental Medicine (Hungary); Pál Maák, Budapest Univ. of Technology and Economics (Hungary)
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A freely triggerable 766nm laser with optimized performance for STED applications
Paper 9329-98
Author(s): Thomas Schönau, Dietmar Klemme, Marcelle König, Benedikt Krämer, Felix Koberling, Kristian Lauritsen, Rainer Erdmann, PicoQuant GmbH (Germany)
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Sarcomere-length variations during sarcomeric assembly and maturation
Paper 9329-99
Author(s): Zhonghai Wang, Siyu Ma, Huaxiao Yang, Clemson Univ. (United States); Thomas K. Borg, The Medical Univ. of South Carolina (United States); Bruce Z. Gao, Clemson Univ. (United States)
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Adaptation of commercial microscopes for advanced imaging applications
Paper 9329-100
Author(s): Craig Brideau, Peter Stys, Univ. of Calgary (Canada)
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All fiber based, multi-color two photon excitation microscopy with an amplified laser diode
Paper 9329-101
Author(s): Sebastian Karpf, Matthias Eibl, Ludwig-Maximilians-Univ. München (Germany); Robert A. Huber, Ludwig-Maximilians-Univ. (Germany), Univ. zu Lübeck (Germany)
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Laser scanning stereomicroscopy for fast volume imaging with two-photon excitation and scanned Bessel beams
Paper 9329-102
Author(s): Tong Ye, Clemson Univ. (United States); Yanlong Yang, Xing Zhou, Xi'an Institute of Optics and Precision Mechanics (China)
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Applied 3D printing for microscopy in health science research
Paper 9329-103
Author(s): Craig Brideau, Kourosh Zareinia, Peter Stys, Univ. of Calgary (Canada)
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Two photon microscopy of native fluorophores spatial location in cancer and non-cancer cell lines
Paper 9329-104
Author(s): Lin Zhang, Lingyan Shi, Robert R. Alfano, The City College of New York (United States)
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TICO Raman: time encoded Raman spectroscopy and microscopy with rapidly wavelength swept lasers
Paper 9329-105
Author(s): Sebastian Karpf, Ludwig-Maximilians-Univ. München (Germany); Matthias Eibl, Ludwig-Maximilians-Univ. München (Germany), Univ. zu Lübeck (Germany); Wolfgang Wieser, Ludwig-Maximilians-Univ. München (Germany); Thomas Klein, Ludwig-Maximilians-Univ. München (Germany); Robert A. Huber, Ludwig-Maximilians-Univ. München (Germany), Univ. zu Lübeck (Germany)
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Imaging how the proteome respond under cell stress by stimulated Raman scattering microscopy
Paper 9329-106
Author(s): Lu Wei, Wei Min, Columbia Univ. (United States)
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Monitoring metabolic enzyme activity in cells with fluorescence lifetime imaging of NAD(P)H
Paper 9329-107
Author(s): Joe T. Sharick, Melissa C. Skala, Vanderbilt Univ. (United States)
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The experimental setup for study biological objects using the methods of femtosecond and CARS microscopy
Paper 9329-108
Author(s): Arseny Aybush, Fedor Gostev, Alexander Zalessky, N.N. Semenov Institute of Chemical Physics (Russian Federation); Konstantin Vereschagin, A. M. Prokhorov General Physics Institute (Russian Federation); Viktor Nadtochenko, N.N. Semenov Institute of Chemical Physics (Russian Federation)
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Detecting the collagen-based hydrogels degradation by multiphoton microscopy (MPM)
Paper 9329-109
Author(s): Xuye Lang, Matthew J. Spousta, Julia G. Lyubovitsky, Univ. of California, Riverside (United States)
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Parallel multiplexed FLIM-FRET imaging in deep tissue of live embryos
Paper 9329-110
Author(s): Ming Zhao, College of Optical Sciences, The Univ. of Arizona (United States); XiaoYang Wan, Univ. of Michigan (United States); Yu Li, College of Optical Sciences, The Univ. of Arizona (United States); Weibin Zhou, Univ. of Michigan (United States); Leilei Peng, College of Optical Sciences, The Univ. of Arizona (United States)
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In vivo non-invasive multiphoton tomography of human skin with subcellular spatial and picosecond time resolution to detect bio- and chemohazards
Paper 9329-111
Author(s): Karsten König, Univ. des Saarlandes (Germany), JenLab GmbH (Germany)
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Two-photon photoacoustic optical imaging using femtosecond pulses
Paper 9329-112
Author(s): Ben E. Urban, Northwestern Univ. (United States)
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Non-invasive discrimination between pancreatic islets and exocrine cells using multiphoton microscopy
Paper 9329-113
Author(s): Binlin Wu, Ge Li, Mingming Hao, Shuibing Chen, Sushmita Mukherjee, Weill Cornell Medical College (United States)
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Imaging cardiomyocytes in intact tissue with a remote focusing microscope
Paper 9329-114
Author(s): Alexander D. Corbett, Rebecca A. B. Burton, Gil Bub, Tony Wilson, Univ. of Oxford (United Kingdom)
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In vivo high-speed imaging of blood cells and tissues in mouse ear skin using multimodal nonlinear optical microscopy
Paper 9329-115
Author(s): Eung Jang Lee, Daeyeon Kim, Bo Ram Kim, Sung-Ho Lee, Seung-Han Park, Yonsei Univ. (Korea, Republic of)
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Investigating cell membrane structure and dynamics with TCSPC-based FLIM
Paper 9329-141
Author(s): Alix Le Marois, Dylan M. Owen, Aleksandar Ivetic, Klaus Suhling, King's College London (United Kingdom)
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Advances in microsecond wide-field TCSPC microscopy
Paper 9329-142
Author(s): Liisa M. Hirvonen, King's College London (United Kingdom); Zdenek Petrasek, Max Planck Institute of Biochemistry (Germany); Andrew Beeby, Durham Univ. (United Kingdom); Klaus Suhling, King's College London (United Kingdom)
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Monday 9 February Show All Abstracts
Session 5:
Technology Development and Application II
Monday 9 February 2015
8:30 AM - 9:35 AM
Multiphoton tomography on astronauts (Invited Paper)
Paper 9329-23
Author(s): Karsten König, JenLab GmbH (Germany), Univ. des Saarlandes (Germany); Martin Weinigel, JenLab GmbH (Germany); Nicole Gerlach, Ulrike Heinrich, DermaTronnier GmbH & Co. KG (Germany)
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Targeted illumination in 3D for excitation and stimulation
Paper 9329-24
Author(s): Elijah Y. Yew, Peter T. C. So, Massachusetts Institute of Technology (United States)
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Breast cancer staging through nonlinear endomicroscopy
Paper 9329-25
Author(s): Gunnsteinn Hall, Wenxuan Liang, Lu Jiang, Kristine Glunde, Xingde Li, Johns Hopkins Univ. (United States)
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Study of acetowhitening mechanisms in live mammalian cells with label-free subcellular-level multimodal nonlinear optical microscopy
Paper 9329-26
Author(s): Jian Lin, Sengkhoon Teh, Wei Zheng, Zi Wang, Zhiwei Huang, National Univ. of Singapore (Singapore)
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JenLab Young Investigator Award Papers Presentation
Monday 9 February 2015
9:35 AM - 10:05 AM
Session 6:
Technology Development and Application III
Monday 9 February 2015
10:30 AM - 12:05 PM
  • Session Chair:
  • Peter T. So, Massachusetts Institute of Technology (United States)
Optical reprogramming with ultrashort femtosecond laser pulses (Invited Paper)
Paper 9329-27
Author(s): Aisada Uchugonova, Univ. des Saarlandes (Germany); Hans Georg Breunig, Univ. des Saarlandes (Germany), JenLab GmbH (Germany); Ana Batista, Karsten König, Univ. des Saarlandes (Germany)
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Compact fixed wavelength femtosecond oscillators for multi-photon imaging
Paper 9329-28
Author(s): Tommi Hakulinen, Spectra Physics (Austria); Ruben Zadoyan, Tommaso Baldacchini, Newport Corp. (United States); Tilman Franke, FEI Munich GmbH (Germany)
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Novel applications of melanin pump-probe imaging: correlating structural and chemical variation with MC1R gene expression and the onset of Parkinson's disease
Paper 9329-29
Author(s): Sanghamitra Deb, Christopher P. Dall, Francisco E. Robles, Christina S. Gainey, Jesse W. Wilson, Duke Univ. (United States); Nicole A. Kukutsch, Leiden Univ. Medical Ctr. (Netherlands); Warren S. Warren, Duke Univ. (United States)
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Femtosecond pulse shaping enables molecular imaging using optical Kerr-effect dynamics
Paper 9329-30
Author(s): Francisco E. Robles, Martin C. Fischer, Warren S. Warren, Duke Univ. (United States)
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Integration of femtosecond fiber laser and endoscopy
Paper 9329-31
Author(s): Huda Yousif, Hussein E. Kotb, Majid Naji, Univ. of Ottawa (Canada); Mohamed A. Abdelalim, Univ. of Ottawa (Canada), Electronics Research Institute (Egypt); Sangeeta Murugkar, Carleton Univ. (Canada); Hanan Anis, Univ. of Ottawa (Canada)
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Multiphoton light-sheet microscopy using wavelength mixing: fast multicolor imaging of the beating Zebrafish heart with low photobleaching
Paper 9329-32
Author(s): Pierre Mahou, Lab. d’Optique et Biosciences, Ecole Polytechnique (France), Univ. of Cambridge (United Kingdom); Julien Vermot, Univ. de Strasbourg (France); Emmanuel Beaurepaire, Lab. d'Optique et Biosciences, Ecole Polytechnique (France); Willy Supatto, Lab. d’Optique et Biosciences, Ecole Polytechnique (France)
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Lunch Break 12:05 PM - 1:30 PM
Session 7:
SHG/THG Microscopy I
Monday 9 February 2015
1:30 PM - 2:50 PM
Combined wavelength dependent 3D SHG imaging, optical scattering and texture analysis to characterize changes collagen architecture and development of a classification system in human ovarian cancer (Invited Paper)
Paper 9329-33
Author(s): Paul J. Campagnola, Karissa B. Tilbury, Kirby R. Campbell, Bruce L. Wen, Kevin W. Eliceiri, Vikas Singh, Manish Patankar, Univ. of Wisconsin-Madison (United States)
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Two- and three-photon excited fluorescence microscopy of erythrocytes using sub-50fs Ti:sapphire and Yb lasers
Paper 9329-34
Author(s): Marcos M. Dantus, Ilyas Saytashev, Michigan State Univ. (United States)
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Combining wide-field assessment with nonlinear optical microscopy: possibilities for multimodal imaging in epithelial (pre)cancer
Paper 9329-35
Author(s): Rahul Pal, Jinping Yang, Gracie Vargas, The Univ. of Texas Medical Branch (United States)
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Four wave mixing microscopy: a high potential nonlinear imaging method
Paper 9329-36
Author(s): Tobias Ehmke, Andreas Knebl, Friedrich-Schiller-Univ. Jena (Germany); Alexander Heisterkamp, Leibniz Univ. Hannover (Germany)
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Identifying different stages during development of colorectal adenocarcinoma using multiphoton microscopy
Paper 9329-37
Author(s): Jianxin Chen, Fujian Normal Univ. (China); Zhifen Chen, Changyin Feng, Guoxian Guan, The Affiliated Union Hospital, Fujian Medical Univ. (China); Lianhuang Li, Fujian Normal Univ. (China)
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Award Presentation
Monday 9 February 2015
2:50 PM - 3:05 PM



Student Poster Session Competition

Prize donated by:
Becker and Hickl, Carl Zeiss Microscopy, LLC, Chroma Tech, Coherent Inc., ISS Inc., Leica Microsystems, Newport Corporation, Princeton Instruments, Semrock, Veroptics

Graduate students and postdoctoral fellows with accepted posters can participate in the poster session competition of the conference on Multiphoton Microscopy in the Biomedical Sciences. There is a cash award for the winner(s). The participants should mention that their submission is for the “Students Poster Session Competition (SPSCMP).” The participants should follow the rules and regulations of SPIE or submission of their abstract and manuscript.


JenLab Young Investigator Award

Prize donated by:
JenLab GmbH

We are pleased to announce that a prize in the amount of $2000.00 will be awarded to a graduate student, postdoc, or scientist under the age of 32 within conference 9329: Multiphoton Microscopy in the Biomedical Sciences XV. To be eligible to receive the award, participants must 1) be both the primary author and presenter of an accepted abstract, and 2) the associated proceedings paper must be submitted at least 2 weeks prior to the meeting start dates for review by the selection committee.
Session 8:
SHG/THG Microscopy II
Monday 9 February 2015
3:30 PM - 5:35 PM
Label-free second order susceptibility imaging of collagen (Invited Paper)
Paper 9329-38
Author(s): Chen-Yuan Dong, Chiu-Mei Hsueh, Ya-Der Huang, Hsuan-Shu Lee, National Taiwan Univ. (Taiwan)
Show Abstract
Influence of pulse width on photodamage and inflammation during intravital autofluorescence 2-photon microscopy of murine intestinal mucosa
Paper 9329-39
Author(s): Gereon Hüttmann, Lisa Krapf, Regina Orzekowsky-Schroeder, Norbert Koop, Alfred Vogel, Antje Klinger, Univ. zu Lübeck (Germany)
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Yb-fiber laser for multimodal imaging
Paper 9329-40
Author(s): Ilyas Saytashev, Michigan State Univ. (United States); Bingwei Xu, Marcos M. Dantus, Biophotonic Solutions, Inc. (United States)
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Label-free multi-photon imaging of Barrett esophagus
Paper 9329-41
Author(s): Seyed Soroush Mehravar, College of Optical Sciences, The Univ. of Arizona (United States); Philip Usera, Hemant Chatrath, Nasser Peyghambarian, College of Optical Sciences, The Univ. of Arizona (United States); Bhaskar Banerjee, The Univ. of Arizona College of Medicine (United States); Khanh Q. Kieu, College of Optical Sciences, The Univ. of Arizona (United States)
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Non-linear imaging and characterization of atherosclerotic arterial tissue using combined SHG and FLIM microscopy
Paper 9329-42
Author(s): Riccardo Cicchi, Istituto Nazionale di Ottica (Italy), European Lab. for Non-linear Spectroscopy (Italy); Christian Matthaeus, Leibniz-Institut für Photonische Technologien e.V. (Germany); Enrico Baria, Univ. degli Studi di Firenze (Italy); Marta Lange, European Lab. for Non-linear Spectroscopy (Italy); Tobias Meyer, Leibniz-Institut für Photonische Technologien e.V. (Germany); Annika Lattermann, Universitätsklinikum Jena (Germany); Benjamin Dietzek, Leibniz-Institut für Photonische Technologien e.V. (Germany), Abbe Ctr. of Photonics Jena (Germany), Friedrich-Schiller-Univ. Jena (Germany); Bernhard R. Brehm, Katholische Klinikum Koblenz (Germany); Juergen Popp, Leibniz-Institut für Photonische Technologien e.V. (Germany), Abbe Ctr. of Photonics Jena (Germany), Friedrich-Schiller-Univ. Jena (Germany); Francesco S. Pavone, European Lab. for Non-linear Spectroscopy (Italy), Univ. degli Studi di Firenze (Italy)
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Biomaterial discrimination using wavelength-dependent SHG excitation efficiency
Paper 9329-43
Author(s): Stephen J. Matcher, Nicola H. Green, Robin M. Delaine-Smith, Gwendolen C. Reilly, Hannah Askew, Xuesong Hu, The Univ. of Sheffield (United Kingdom)
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Discontinuous sampling in beam-scanning microscopy
Paper 9329-44
Author(s): Ryan D. Muir, Paul D. Schmitt, Emma L. DeWalt, Shane Z. Sullivan, Ximeng You, Garth J. Simpson, Purdue Univ. (United States)
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Second harmonic generation imaging in tissue engineering and cartilage pathologies
Paper 9329-45
Author(s): Magnus B. Lilledahl, Norwegian Univ. of Science and Technology (Norway); Magnus Ø. Olderøy, Norwegian Ctr. for Stem Cell Research (Norway); Andreas Finnøy, Norwegian Univ. of Science and Technology (Norway); Kristin Olstad, Norwegian Univ. of Life Sciences (Norway); Jan E. Brinchman, Norwegian Ctr. for Stem Cell Research (Norway)
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Tuesday 10 February Show All Abstracts
Session 9:
Biomedical Applications of Coherent Raman I
Tuesday 10 February 2015
8:00 AM - 9:55 AM
Stimulated Raman scattering microscopy of chemical tags in live cells (Invited Paper)
Paper 9329-46
Author(s): YanYi Huang, Peking Univ. (China); Xing Chen, Peking Univ (China)
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Bioorthogonal vibrational imaging of dynamic metabolism of living systems (Invited Paper)
Paper 9329-47
Author(s): Wei Min, Columbia Univ. (United States)
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Visualization and quantification of peripheral myelin degeneration in amyotrophic lateral sclerosis (ALS) mouse model (SOD1G93A) and patients with stimulated Raman scattering (SRS)
Paper 9329-48
Author(s): Wenlong Yang, Feng Tian, Harvard Univ. (United States); Daniel Mordes, Harvard Univ. (United States), Massachusetts General Hospital (United States); Naoki Suzuki, Satomi Suzuki, Kevin Eggan, Xiaoliang S. Xie, Harvard Univ. (United States)
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Probing myelin morphology and organization with label-free microscopy
Paper 9329-49
Author(s): Alicja Gasecka, Institut Univ. en Santé Mentale de Quebec, Univ. Laval (Canada), Institut Fresnel (Canada); Pierre Gravel, Institute Univ. en Santé Mentale de Quebec, Univ. Laval (Canada), Univ. du Québec (Canada); Raphael Turcotte, Wellman Ctr. for Photomedicine (United States); Steve Begin, Daniel Cote, Ctr. de Recherche de l'Univ. Laval Robert-Giffard (Canada), Institut Univ. en Santé Mentale de Quebec, Univ. Laval (Canada)
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Visualizing murine meibomian glands physiology and pathology using coherent anti-stokes Raman scattering (CARS) microscopy and multi-photon microscopy (MPM)
Paper 9329-50
Author(s): Joachim Pruessner, Univ. zu Lübeck (Germany), Wellman Ctr. for Photomedicine (United States); Reginald Birngruber, Univ. zu Lübeck (Germany); Conor L. Evans, Wellman Ctr. for Photomedicine (United States)
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Local organization of lipids in myelinated axons probed by polarization resolved coherent anti-stokes Raman scattering nonlinear microscopy
Paper 9329-51
Author(s): Paulina Gasecka, Institut Fresnel (France)
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Monitoring lipid accumulation in the green microalga botryococcus braunii with frequency-modulated stimulated Raman scattering
Paper 9329-52
Author(s): Chun-Chin Wang, Dayananda Chandrappa, Nicholas Smirnoff, Julian Moger, Univ. of Exeter (United Kingdom)
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Session 10:
Biomedical Applications of Coherent Raman II
Tuesday 10 February 2015
10:25 AM - 12:00 PM
  • Session Chair:
  • Daniel Cote, Ctr. de Recherche de l'Univ. Laval Robert-Giffard (Canada)
Label-free functional imaging of membrane potential by stimulated Raman scattering microscopy (Invited Paper)
Paper 9329-53
Author(s): Bin Liu, Key Lab. of Science and Technology on Tunable Laser, Harbin Institute of Technology (China); Hyeon Jeong Lee, Interdisciplinary Life Science Program, Purdue Univ. (United States); Delong Zhang, ChienSheng Liao, Purdue Univ. (United States); Yuanqin Xia, Key Lab. of Science and Technology on Tunable Laser, Harbin Institute of Technology (China); Ji-Xin Cheng, Purdue Univ. (United States)
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Detection of human brain tumors with SRS microscopy
Paper 9329-54
Author(s): Daniel A. Orringer, Univ. of Michigan Health System (United States); Minbiao Ji, Harvard Univ. (United States); Spencer Lewis, Sandra Camelo-Piragua, Univ. of Michigan Health System (United States); Xiaoliang S. Xie, Harvard Univ. (United States)
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Imaging human melanoma using coherent anti-stokes Raman scattering (CARS) and multiphoton microscopy (MPM)
Paper 9329-55
Author(s): Hequn Wang, Sam Osseiran, Elisabeth Roider, Vivien Igras, David E. Fisher, Conor L. Evans, Wellman Ctr. for Photomedicine (United States)
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Histology in vivo: chemical contrast combined with clinical multimodal multiphoton tomograpy
Paper 9329-56
Author(s): Martin Weinigel, JenLab GmbH (Germany), Univ. of Technology, Ilmenau (Germany); Hans Georg Breunig, Saarland Univ. (Germany), JenLab GmbH (Germany); Karsten Koenig, JenLab GmbH (Germany), Univ. des Saarlandes (Germany)
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Mechanically-induced protein structural changes in fibrin hydrogels using B-CARS microscopy
Paper 9329-57
Author(s): Sapun H. Parekh, Frederik Fleissner, Mischa Bonn, Max-Planck-Institut für Polymerforschung (Germany)
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Simultaneous quadruple modal nonlinear optical imaging for gastric diseases diagnosis and characterization
Paper 9329-58
Author(s): Zi Wang, Wei Zheng, Jian Lin, Zhiwei Huang, National Univ. of Singapore (Singapore)
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Lunch Break 12:00 PM - 1:30 PM
Session 11:
Coherent Raman Technical Development I
Tuesday 10 February 2015
1:30 PM - 3:25 PM
  • Session Chair:
  • Jixin Cheng, Univ. of Science and Technology of China (China)
Hyperspectral stimulated Raman scattering imaging of drug-cell and drug-drug interaction (Invited Paper)
Paper 9329-59
Author(s): Dan Fu, Harvard Univ. (United States); Jing Zhou, Novartis Institutes for Biomedical Research, Inc. (United States); Xiaoliang S. Xie, Harvard Univ. (United States)
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In vivo microsecond scale vibrational spectroscopy imaging by modulation multiplexed stimulated Raman scattering (Invited Paper)
Paper 9329-60
Author(s): ChienSheng Liao, Pu Wang, Ping Wang, Robert Oglesbee, Ji-Xin Cheng, Purdue Univ. (United States)
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Resolution and contrast enhancement in label-free microscopy
Paper 9329-61
Author(s): Alicja Gasecka, Institut Univ. en Santé Mentale de Quebec, Univ. Laval (Canada), Institut Fresnel (Canada); Amy Daradich, Harold Dehez, Institut Univ. en Santé Mentale de Quebec, Univ. Laval (Canada); Michel Piche, Univ. Laval (Canada); Daniel Cote, Ctr. de Recherche de l'Univ. Laval Robert-Giffard (Canada), Institut Univ. en Santé Mentale de Quebec, Univ. Laval (Canada)
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Fast spectrum extraction from SRS spectral images by independent component analysis with image compression and post sampling
Paper 9329-62
Author(s): Yasuyuki Ozeki, The Univ. of Tokyo (Japan)
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Beam scanning approach to broadband coherent Raman imaging
Paper 9329-63
Author(s): Young Jong Lee, Charles H. Camp, Marcus T. Cicerone, National Institute of Standards and Technology (United States)
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Nonlinear optical microscopy and optical coherence tomography platform for label-free functional biophotonic imaging
Paper 9329-64
Author(s): Angelika Unterhuber, Medizinische Univ. Wien (Austria); Sunil Kumar, Weizmann Institute of Science (Israel); Tschackad Kamali, Boris Hermann, Rene Werkmeister, Medizinische Univ. Wien (Austria); Yaron Silberberg, Weizmann Institute of Science (Israel); Wolfgang Drexler, Medizinische Univ. Wien (Austria)
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CARS/FWM and scanning probe microscopy of nanostructures (Invited Paper)
Paper 9329-65
Author(s): Annika M. Enejder, Michael Stührenberg, Nisha Rani Agarwal, Mahesh Namboodiri, Juris Kiskis, Chalmers Univ. of Technology (Sweden)
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Session 12:
Coherent Raman Technical Development II
Tuesday 10 February 2015
3:55 PM - 5:50 PM
  • Session Chair:
  • Marcus T. Cicerone, National Institute of Standards and Technology (United States)
Background-free stimulated Raman spectroscopy and microscopy (Invited Paper)
Paper 9329-66
Author(s): Hervé Rigneault, Esben Andresen, Pascal Berto, Institut Fresnel (France)
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Pushing the limits of coherent Raman spectroscopic imaging (Invited Paper)
Paper 9329-67
Author(s): Marcus T. Cicerone, Charles H. Camp, Brandon Blakely, Young Lee, National Institute of Standards and Technology (United States)
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Fiber-laser platform for coherent Raman scattering
Paper 9329-68
Author(s): Christian W. Freudiger, Invenio Imaging Inc. (United States); Wenlong Yang, Gary R. Holtom, Harvard Univ. (United States); Jay Trautman, Invenio Imaging Inc. (United States); Khanh Q. Kieu, The Univ. of Arizona (United States); Sunney Xie, Harvard Univ. (United States)
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Optimized coherent Raman scattering microscopy with a novel tunable dual-wavelength lightsource
Paper 9329-69
Author(s): Ingo Rimke, APE GmbH (Germany); Gregor Hehl, Univ. Stuttgart (Germany); Marcus Beutler, Peter Volz, Edlef Büttner, APE GmbH (Germany); Andreas Volkmer, Univ. Stuttgart (Germany)
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Towards low-noise fiber sources for coherent Raman microscopy
Paper 9329-70
Author(s): Erin S. Lamb, Hanzhang Pei, Frank W. Wise, Cornell Univ. (United States)
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Improved signal-to-noise ratio with shot noise limited detection in SRS microscopy
Paper 9329-71
Author(s): Miriam J. B. Moester, Freek Ariese, Johannes F. de Boer, Vrije Univ. Amsterdam (Netherlands)
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Revealing the uptake mechanisms of polymeric nanomedicines with coherent Raman scattering microscopy
Paper 9329-72
Author(s): Natalie L. Garrett, Univ. of Exeter (United Kingdom); Lisa Godfrey, Univ. College London (United Kingdom); Aikaterini Lalatsa, Univ. of Portsmouth (United Kingdom); Ijeoma F. Uchegbu, Andreas G. Schatzlein, Univ. College London (United Kingdom); Julian Moger, Univ. of Exeter (United Kingdom)
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