The Moscone Center
San Francisco, California, United States
21 - 26 January 2012
Conference 8226
Multiphoton Microscopy in the Biomedical Sciences XII
Sunday - Tuesday 22 - 24 January 2012
Important
Dates
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Abstract Due:
11 July 2011

Author Notification:
19 September 2011

Manuscript Due Date:
21 December 2011

Conference
Committee
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Sunday 22 January Show All Abstracts
Opening Remarks
Sunday 22 January 2012
8:15 AM - 8:30 AM
Session KEY1:
Keynote Session
Sunday 22 January 2012
8:30 AM - 10:00 AM
Innovative microscopes and labels for intravital imaging (Invited Paper)
Paper 8226-1
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Fluorescence lifetime imaging and metrology for cell biology, drug discovery and label-free diagnosis (Invited Paper)
Paper 8226-2
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In vivo deep tissue fluorescence imaging of the murine small intestine and colon (Invited Paper)
Paper 8226-3
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Session 1:
FRET, FLIM, FCS I
Sunday 22 January 2012
10:30 AM - 12:15 PM
Polarized fluorescence correlation spectroscopy (pFCS): a single-molecule method for simultaneously measuring homo-FRET, brightness, and the diffusion of protein complexes in living cells (Invited Paper)
Paper 8226-4
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Dynamic nuclear protein interactions investigated using fluorescence lifetime and fluorescence fluctuation spectroscopy (Invited Paper)
Paper 8226-5
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Spatially resolved recording of transient fluorescence-lifetime effects by line-scanning TCSPC (Invited Paper)
Paper 8226-6
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Phasor FLIM metabolic mapping of stem cells and cancer cells in live tissues
Paper 8226-7
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Fluorescence lifetime imaging microscopy (FLIM) studies of living primary human cells for applications in tissue regeneration
Paper 8226-8
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High speed 3D-resolved fluorescence and phosphorescence heterodyned wide field lifetime imaging
Paper 8226-9
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Session 2:
FRET, FLIM, FCS II
Sunday 22 January 2012
1:25 PM - 3:05 PM
Multiwavelength FLIM: new concept for fluorescence diagnosis (Invited Paper)
Paper 8226-10
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Clinical multiphoton FLIM tomography (Invited Paper)
Paper 8226-11
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Monitoring transient elastic energy storage within the rotary motors of single FoF1-ATP synthase by DCO-ALEX FRET
Paper 8226-12
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Three-color FRET expands the ability to quantify the interactions of several proteins involved in actin filament nucleation
Paper 8226-13
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New detection and analysis FLIM and FCS techniques for confocal laser scanning microscopes
Paper 8226-14
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In vivo, high resolution measurement of cerebral oxygen tension and NADH using phosphorescence- and fluorescence- lifetime imaging
Paper 8226-15
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Session 3:
FRET, FLIM, FCS III
Sunday 22 January 2012
3:30 PM - 5:30 PM
An automated approach for the analysis of multispectral multiphoton FLIM data and its application to the diagnosis of skin cancer
Paper 8226-16
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Multiphoton fluorescence lifetime imaging of cleared mouse organs
Paper 8226-17
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Ion-beam sputtered (IBS) thin-film interference filters for nonlinear optical imaging
Paper 8226-18
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Recent development in ultrafast lasers for multimodal nonlinear imaging
Paper 8226-19
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Latest advances in ultrafast laser sources for multiphoton microscopy
Paper 8226-20
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Pulse shaping multiphoton FRET microscopy
Paper 8226-21
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Analysis of human aorta using fluorescence lifetime imaging microscopy (FLIM)
Paper 8226-22
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Fluorescence lifetime and fluorescence correlation spectroscopy in colloidal quantum dots
Paper 8226-23
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Session PSun2:
Posters - Sunday
Sunday 22 January 2012
5:30 PM - 7:30 PM
Location: Room 103 (Exhibit Level)

Conference attendees are invited to attend the BiOS poster session on Sunday evening. Come view the posters, enjoy light refreshments, ask questions, and network with colleagues in your field. Authors of poster papers will be present to answer questions concerning their papers. Attendees are required to wear their conference registration badges to the poster sessions. Poster authors, view poster presentation guidelines and set-up instructions at http://spie.org/x27476.xml.

Three-color FRET expands the ability to quantify the interactions of several proteins involved in actin nucleation, Horst K. Wallrabe, Yuansheng Sun, Ammasi Periasamy, Xiaolan Fang, George S. Bloom, Univ. of Virginia (United States) [8226 12]
Rapid volumetric temporal focusing multiphoton microscopy of neural activity: theory, image processing, and experimental realization
Paper 8226-82
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In vivo pump-probe microscopy of melanoma and pigmented lesions
Paper 8226-83
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Carcinogenic risk from nonlinear optical imaging: comparison between skin and internal organs tissues
Paper 8226-84
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Second-harmonic generation and fluorescence lifetime imaging microscopy through a rodent mammary imaging window
Paper 8226-85
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Background-free nonlinear microspectroscopy with vibrational molecular interferometry
Paper 8226-86
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Stimulated Raman scattering microscopy by spectral focussing and fiber-generated soliton as Stokes pulse
Paper 8226-87
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In vivo multiphoton imaging of the cornea: polarization-resolved second harmonic generation from stromal collagen
Paper 8226-46
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Studies on wide-field-of-view multiphoton imaging using the flexible clinical multiphoton tomograph MPTflex
Paper 8226-88
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Custom-made Cr: LiCAF laser for a nonlinear microscope
Paper 8226-89
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Quantifying colonic cancer progression with multiphoton microscopy
Paper 8226-90
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Recommendations for the design and the installation of large laser scanning microscopy systems
Paper 8226-91
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The use of microstructures for high-resolution axial axis imaging
Paper 8226-92
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Comparison of calcium imaging in dorsal root ganglion neurons by using laser scanning confocal and two-photon microscopy
Paper 8226-93
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Development of photon detectors for picosecond resolution, high rate, multi-channel life science applications
Paper 8226-94
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Characterization of optical properties of ZnO nanoparticles for quantitative assessment of transdermal transport
Paper 8226-95
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Multifocus nonlinear optical microscopy based on SLM
Paper 8226-96
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Two-photon excited fluorescence spectroscopy and imaging of melanin in vitro and in vivo
Paper 8226-97
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Long-distance fluorescence lifetime imaging with stimulated emission and electronic time delay
Paper 8226-98
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Structural heterogeneities of self-assembled peptide nanomaterials
Paper 8226-99
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Multiphoton ultraviolet microscopy reveals dopamine dynamics in live brain tissue
Paper 8226-100
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Non-invasive monitoring of redox state to assess cell differentiation in engineered adipose tissues
Paper 8226-101
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Investigation of laser-induced cyclobutane-pyrimidin-dimers damage to cellular DNA in nonlinear optical imaging
Paper 8226-102
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Low cost laser system generating 26-fs pulse duration, 30-kW peak power, and tunability from 800 to 1200 nm for multiphoton microscopy
Paper 8226-103
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Correlation scanning microscopy techniques to quantify DNA repair protein kinetics using confocal microscopy and two-photon microscopy
Paper 8226-104
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Photodamage in whole living organisms under imaging conditions as a function of pulse duration
Paper 8226-105
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Design and performance of a wide field-of-view multiphoton microscope
Paper 8226-106
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Real-time action potential recording from multiple neurons
Paper 8226-107
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Random access multiphoton (RAMP) microscopy for investigation of cerebral blood flow regulation mechanisms
Paper 8226-108
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Effect of pulse duration on photodamage in living organisms
Paper 8226-109
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Toward nonlinear microscopy with few-cycle laser pulses
Paper 8226-110
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Sensitive fluorescence detection using a camera from the gaming industry
Paper 8226-111
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Three-photon fluorescence imaging of melanin with a dual-wedge confocal scanning system
Paper 8226-112
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Improvement of the spatial resolution in two-photon excitation fluorescence microscopy by saturated excitation (SAX)
Paper 8226-114
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Totally integrated linear and nonlinear optics multimodal microscopy platform to understand single cell processes
Paper 8226-115
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Automated control of optical polarization for nonlinear microscopy
Paper 8226-116
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Oxygen-glucose deprivation (OGD) induced changes in the microtubules structure of cultured rat cortical neurons' axons studied using polarization SHG imaging
Paper 8226-117
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Probing live samples in second-harmonic generation microscopy using specific markers and fluorescent proteins
Paper 8226-118
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The structural origin of second-harmonic generation in fascia
Paper 8226-119
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Application of second-harmonic generation microscopy for in-vivo observation of structural change in human dermal collagen fiber caused by aging and/or UV exposure
Paper 8226-120
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Second harmonic generation microscopy differentiates collagen type I and type III in COPD
Paper 8226-121
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Second-harmonic generation microscopy used to evaluate the effect of the dimethyl sulfoxide in the cryopreservation process in collagen fibers of differentiated chondrocytes
Paper 8226-122
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Three-dimensional analysis of muscle fiber orientation in a single-shot through polarization-resolved second-harmonic generation holography
Paper 8226-123
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Hilbert-transform phase shifting second-harmonic generation holographic imaging
Paper 8226-124
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Nonlinear optics for the study of human scar tissue
Paper 8226-125
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Multiphoton imaging with 10 fs pulses: comparing signal strength and photo damage
Paper 8226-126
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Breast pathology assessment using two-photon microscopy
Paper 8226-127
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SHG microscopy of articular cartilage to image osteoarthritis
Paper 8226-128
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Use of the second harmonic generation microscopy to evaluate chondrogenic differentiation of mesenchymal stem cells for cartilage repair
Paper 8226-129
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In vivo multiphoton microscopy associated to 3D image processing for human skin characterization
Paper 8226-130
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Quantitative second-harmonic generation imaging to detect osteogenesis imperfecta in human skin samples
Paper 8226-131
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Applications of second-harmonic (SHG) and third-harmonic generation(THG) in diagnostic cytology and histology
Paper 8226-132
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A study on the spectral dependence of second harmonic generation from collagen tissues
Paper 8226-133
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Two-photon imaging and spectroscopy of fresh human colon biopsies
Paper 8226-134
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Characterizing the interactions between prolyl isomerase pin1 and phosphatase inhibitor-2 in living cells with FRET and FCS
Paper 8226-135
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Spectral FLIM detection and new analysis schemes for confocal laser scanning microscopes
Paper 8226-136
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Fluorescence lifetime imaging microscopy with acousto-optic deflectors
Paper 8226-137
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A simple laser Ti:Sapphire system upgrade for multimodal nonlinear optical microscopy
Paper 8226-138
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Integrated multimodality microscope for functional imaging of engineered and natural tissues
Paper 8226-139
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Building an "affordable" coherent Raman scattering (CRS) microscope
Paper 8226-141
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Multicolor stimulated Raman scattering (SRS) microscopy
Paper 8226-142
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Evaluation of atherosclerotic plaque development by texture analysis of multimodal CARS images of rabbit arteries
(Canceled)
Paper 8226-143
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Application of hyperspectral coherent anti-Stokes Raman scattering microscopy to pharmaceutics
Paper 8226-144
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The use of two-photon microscopy to study the biological effects of focused ultrasound on the brain
Paper 8226-145
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Dynamic nuclear protein interactions investigated using FRET-FLIM and FCS
Paper 8226-146
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Monitoring changes in endogenous fluorophores through quantitative FLIM imaging in live cells
Paper 8226-150
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Monday 23 January Show All Abstracts
Session 4:
Technology Development & Applications I
Monday 23 January 2012
8:00 AM - 9:40 AM
Multiphoton tomography in animal research (Invited Paper)
Paper 8226-24
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Watching stem cells at work with a flexible multiphoton tomograph (Invited Paper)
Paper 8226-25
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Spectral phasor analysis allows rapid and easy unmixing of fluorescence microscopy spectral images
Paper 8226-26
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Photon reassignment for structured light imaging based microscopy
Paper 8226-27
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Single-shot 3D multi-photon microscopy
Paper 8226-28
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Multimodal polarimetric nonlinear microscopy in tissues
Paper 8226-29
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Session AWD:
JenLab Young Investigator Award Paper Presentations
Monday 23 January 2012
9:40 AM
Session 5:
Technology Development & Applications II
Monday 23 January 2012
10:40 AM - 12:10 PM
Ray-tracing study on the post-scanner variable beam expansion optics in a two-photon microscopy system
Paper 8226-30
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Scanning fiber optic two-photon excitation endomicroscopy with reduced photo damage and precision focal position localization
Paper 8226-31
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Two-photon imaging based on both tryptophan autofluorescence and exogenous fluorescence
Paper 8226-32
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Targeted nanosensor aided three-dimensional pH mapping in tumor spheroids using two-photon microscopy
Paper 8226-33
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Implementation and characterization of an axicon-based nonlinear digital line-scanning microscope
Paper 8226-34
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Chronic, simultaneous multiphoton imaging of cortical layers I to V with microprism implants
Paper 8226-35
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Session 6:
Harmonic Generation Microscopy I
Monday 23 January 2012
1:15 PM - 3:05 PM
Polarization resolved SHG as a robust means to quantify changes in collagen in fibrotic diseases (Invited Paper)
Paper 8226-36
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Analysis of collagen structure changes in ovarian tumor development
Paper 8226-37
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In vivo characterization of organizational and orientation changes in precancerous submucosal collagen via polarization SHG
Paper 8226-38
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Polarization-resolved SHG microscopy of collagenous tissues with controlled mechanical strain
Paper 8226-39
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Combined nonlinear laser imaging (two-photon excitation fluorescence, second and third-harmonic generation, and fluorescence lifetime imaging microscopies) in ovarian tumors
Paper 8226-40
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Third-harmonic (THG) and four-wave mixing microscopy (FWM) of cells and biological tissues
Paper 8226-41
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Second-harmonic generation endomicroscopy imaging system for preterm birth imaging
Paper 8226-42
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Session AWD:
Award Presentations
Monday 23 January 2012
3:05 PM

JenLab Young Investigator Award

Best Student Poster Awards
Session 7:
Harmonic Generation Microscopy II
Monday 23 January 2012
3:35 PM - 5:50 PM
Studies of human skin glycation by the use of multiphoton microscopy and spectroscopy
Paper 8226-43
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High-speed vibrational sum frequency generation microscopy
Paper 8226-44
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Generic model for the biomolecular organization probed by second-harmonic generation polarization microscopy
Paper 8226-45
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Investigation of the wavelength dependence of SHG from various tissues
Paper 8226-47
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Adaptive multiphoton and harmonic generation microscopy for whole tissue imaging
(Canceled)
Paper 8226-48
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Multiphoton imaging with sub-30 fs Yb fiber laser
Paper 8226-49
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Video-rate, label-free second-harmonic generation holographic imaging of biological specimens
Paper 8226-50
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Structure of collagen fibers revealed with polarization dependent second-harmonic generation microscopy
Paper 8226-51
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Coupling two-photon excitation with optical nanoscopy and light-sheet illumination microscopy
Paper 8226-147
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Tuesday 24 January Show All Abstracts
Session 8:
Hyperspectral Imaging with CRS
Tuesday 24 January 2012
8:00 AM - 10:05 AM
Lock-in free stimulated Raman microscopy (Invited Paper)
Paper 8226-52
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High-speed spectral tuning CARS microscopy using AOTF laser
Paper 8226-53
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Ultra-broadband time-resolved coherent anti-Stokes Raman scattering microspectroscopy
Paper 8226-54
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Broadband CARS microscopy: phenotypic and functional imaging for biology
Paper 8226-55
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High-resolution high-speed tunable grating filter for stimulated Raman spectral imaging
Paper 8226-56
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A spectrally modulated stimulated Raman scattering microscope
Paper 8226-57
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From coherent Raman microscopy to quantitative coherent Raman spectral imaging (Invited Paper)
Paper 8226-58
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Session 9:
Applications of CRS Microscopy
Tuesday 24 January 2012
10:25 AM - 12:35 PM
CARS for catalysis (Invited Paper)
Paper 8226-59
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CARS spectro-microscopy of cell patterns in Drosophila melanogaster wing imaginal disc
Paper 8226-60
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Coherent Raman scattering microscopy for label-free imaging of live amphioxus
Paper 8226-61
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Neuronal cell growth on polymeric scaffolds studied by CARS microscopy
Paper 8226-62
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Study of signal relay and chemotaxis of neutrophils using broadband CARS microscopy
Paper 8226-63
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Live animal nervous system imaging with video-rate multimodal coherent anti-Stokes Raman scattering microendoscopy
Paper 8226-64
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Surface-mediated four-wave mixing microscopy (Invited Paper)
Paper 8226-65
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Towards a tunable fiber source for coherent Raman imaging
Paper 8226-148
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Session 10:
CRS for Medical Diagnostics
Tuesday 24 January 2012
1:40 PM - 3:00 PM
Early demyelinating lesions of the nervous system: a multimodal approach with CARS imaging (Invited Paper)
Paper 8226-66
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Chemically selective spectral imaging of bone mineral density and the collagen manifold in bulk bone using CARS and SHG
Paper 8226-67
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Clinical multiphoton and CARS microscopy
Paper 8226-68
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In vivo monitoring specialized hepatocyte-like cells in Drosophila by coherent anti-Stokes Raman scattering (CARS) and two-photon excitation fluorescence (TPE-F) microscopy
Paper 8226-69
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An integrated coherent anti-Stokes Raman scattering and multiphoton imaging technique for liver disease diagnosis
Paper 8226-70
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Session 11:
Fiber Laser Technology for CRS
Tuesday 24 January 2012
3:20 PM - 4:30 PM
All-fiber tunable picosecond laser source for coherent Raman scattering microscopy (Invited Paper)
Paper 8226-71
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Active and passive photonic crystal fibers for compact coherent Raman scattering (CRS) microscope and endoscopes
Paper 8226-72
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Fiber delivered two-color picosecond source for coherent Raman scattering imaging (Invited Paper)
Paper 8226-73
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A novel multimodal CARS miniaturized microscope
Paper 8226-74
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Session 12:
Advanced CRS Microscopy Instrumentation
Tuesday 24 January 2012
4:30 PM - 6:15 PM
SLM-aided wide-field CARS microscopy
Paper 8226-75
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Computational optimization of phase shaped CARS
Paper 8226-76
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Stimulated Raman scattering based multimodal nonlinear optical microscopy
(Canceled)
Paper 8226-77
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In-vivo SRS microscopy in biomedical applications by using longer wavelength excitation
Paper 8226-78
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Parallel CARS and OCT imaging
Paper 8226-79
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Simple and robust interferometric non-resonant background removal for broadband coherent anti-Stokes Raman scattering
(Canceled)
Paper 8226-80
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Phase contrast in wide-field coherent anti-Stokes Raman scattering (CARS) microscopy
Paper 8226-81
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